Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24000 and BPHYT_RS24005 are separated by 102 nucleotides BPHYT_RS24005 and BPHYT_RS24010 are separated by 14 nucleotides BPHYT_RS24010 and BPHYT_RS24015 are separated by 35 nucleotides
BPHYT_RS24000: BPHYT_RS24000 - L-histidine ABC transporter, substrate-binding component (from data) , at 950,479 to 951,339
_RS24000
BPHYT_RS24005: BPHYT_RS24005 - L-histidine ABC transporter, permease component 1 (from data) , at 951,442 to 952,164
_RS24005
BPHYT_RS24010: BPHYT_RS24010 - L-histidine ABC transporter, permease component 2 (from data) , at 952,179 to 952,931
_RS24010
BPHYT_RS24015: BPHYT_RS24015 - L-histidine ABC transporter, ATPase component (from data) , at 952,967 to 953,758
_RS24015
Position (kb)
951
952
953 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 950.728 kb on - strand, within BPHYT_RS24000 at 951.121 kb on + strand, within BPHYT_RS24000 at 951.125 kb on - strand, within BPHYT_RS24000 at 951.436 kb on + strand at 951.491 kb on + strand at 951.622 kb on + strand, within BPHYT_RS24005 at 951.622 kb on + strand, within BPHYT_RS24005 at 951.791 kb on - strand, within BPHYT_RS24005 at 952.039 kb on + strand, within BPHYT_RS24005 at 952.467 kb on + strand, within BPHYT_RS24010 at 952.467 kb on + strand, within BPHYT_RS24010 at 952.791 kb on + strand, within BPHYT_RS24010
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 950,728 - BPHYT_RS24000 0.29 +0.2 951,121 + BPHYT_RS24000 0.75 -0.7 951,125 - BPHYT_RS24000 0.75 -1.2 951,436 + +2.2 951,491 + -1.0 951,622 + BPHYT_RS24005 0.25 +1.2 951,622 + BPHYT_RS24005 0.25 -2.3 951,791 - BPHYT_RS24005 0.48 -0.8 952,039 + BPHYT_RS24005 0.83 -1.6 952,467 + BPHYT_RS24010 0.38 +0.7 952,467 + BPHYT_RS24010 0.38 +0.5 952,791 + BPHYT_RS24010 0.81 +0.2
Or see this region's nucleotide sequence