Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23985 and BPHYT_RS23990 are separated by 405 nucleotides BPHYT_RS23990 and BPHYT_RS23995 are separated by 12 nucleotides BPHYT_RS23995 and BPHYT_RS24000 are separated by 293 nucleotides
BPHYT_RS23985: BPHYT_RS23985 - carbohydrate porin, at 946,724 to 948,247
_RS23985
BPHYT_RS23990: BPHYT_RS23990 - N-hydroxyarylamine O-acetyltransferase, at 948,653 to 949,492
_RS23990
BPHYT_RS23995: BPHYT_RS23995 - isoprenylcysteine carboxyl methyltransferase, at 949,505 to 950,185
_RS23995
BPHYT_RS24000: BPHYT_RS24000 - L-histidine ABC transporter, substrate-binding component <small>(from data)</small>, at 950,479 to 951,339
_RS24000
Position (kb) 948
949
950 Strain fitness (log2 ratio) -1
0
1 at 947.772 kb on - strand, within BPHYT_RS23985 at 947.772 kb on - strand, within BPHYT_RS23985 at 947.941 kb on - strand, within BPHYT_RS23985 at 948.361 kb on - strand at 948.424 kb on - strand at 948.446 kb on + strand at 948.446 kb on + strand at 948.467 kb on - strand at 948.483 kb on - strand at 948.676 kb on + strand at 949.185 kb on - strand, within BPHYT_RS23990 at 949.901 kb on + strand, within BPHYT_RS23995 at 949.901 kb on + strand, within BPHYT_RS23995 at 949.943 kb on - strand, within BPHYT_RS23995 at 949.989 kb on - strand, within BPHYT_RS23995 at 950.078 kb on - strand, within BPHYT_RS23995 at 950.141 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 947,772 - BPHYT_RS23985 0.69 -0.8 947,772 - BPHYT_RS23985 0.69 -0.2 947,941 - BPHYT_RS23985 0.80 -0.6 948,361 - +1.5 948,424 - +0.2 948,446 + -0.2 948,446 + -0.4 948,467 - -0.5 948,483 - -0.1 948,676 + +0.2 949,185 - BPHYT_RS23990 0.63 +0.8 949,901 + BPHYT_RS23995 0.58 -1.4 949,901 + BPHYT_RS23995 0.58 -0.2 949,943 - BPHYT_RS23995 0.64 -0.4 949,989 - BPHYT_RS23995 0.71 -0.8 950,078 - BPHYT_RS23995 0.84 -0.2 950,141 + +0.8
Or see this region's nucleotide sequence