Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23255 and BPHYT_RS23260 are separated by 306 nucleotides BPHYT_RS23260 and BPHYT_RS23265 are separated by 49 nucleotides
BPHYT_RS23255: BPHYT_RS23255 - TetR family transcriptional regulator, at 781,278 to 782,027
_RS23255
BPHYT_RS23260: BPHYT_RS23260 - isovaleryl-CoA dehydrogenase, at 782,334 to 783,515
_RS23260
BPHYT_RS23265: BPHYT_RS23265 - methylcrotonoyl-CoA carboxylase, at 783,565 to 785,172
_RS23265
Position (kb) 782
783
784 Strain fitness (log2 ratio) -2
-1
0
1 at 781.357 kb on - strand, within BPHYT_RS23255 at 781.417 kb on - strand, within BPHYT_RS23255 at 782.028 kb on - strand at 782.121 kb on + strand at 782.162 kb on + strand at 782.547 kb on + strand, within BPHYT_RS23260 at 782.680 kb on - strand, within BPHYT_RS23260 at 782.680 kb on - strand, within BPHYT_RS23260 at 782.793 kb on + strand, within BPHYT_RS23260 at 782.794 kb on - strand, within BPHYT_RS23260 at 783.142 kb on - strand, within BPHYT_RS23260 at 783.217 kb on - strand, within BPHYT_RS23260 at 783.217 kb on - strand, within BPHYT_RS23260 at 783.331 kb on - strand, within BPHYT_RS23260 at 783.394 kb on - strand, within BPHYT_RS23260 at 783.442 kb on - strand at 783.563 kb on + strand at 783.564 kb on - strand at 783.808 kb on + strand, within BPHYT_RS23265 at 783.809 kb on - strand, within BPHYT_RS23265 at 783.913 kb on + strand, within BPHYT_RS23265 at 784.068 kb on + strand, within BPHYT_RS23265 at 784.144 kb on + strand, within BPHYT_RS23265 at 784.278 kb on + strand, within BPHYT_RS23265 at 784.433 kb on - strand, within BPHYT_RS23265
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 781,357 - BPHYT_RS23255 0.11 +0.4 781,417 - BPHYT_RS23255 0.19 -0.3 782,028 - -0.2 782,121 + +0.4 782,162 + -0.4 782,547 + BPHYT_RS23260 0.18 -1.1 782,680 - BPHYT_RS23260 0.29 +0.8 782,680 - BPHYT_RS23260 0.29 -0.4 782,793 + BPHYT_RS23260 0.39 +1.1 782,794 - BPHYT_RS23260 0.39 +0.9 783,142 - BPHYT_RS23260 0.68 +1.4 783,217 - BPHYT_RS23260 0.75 +0.5 783,217 - BPHYT_RS23260 0.75 -0.2 783,331 - BPHYT_RS23260 0.84 +0.3 783,394 - BPHYT_RS23260 0.90 +0.2 783,442 - +0.8 783,563 + -0.0 783,564 - +0.1 783,808 + BPHYT_RS23265 0.15 -0.6 783,809 - BPHYT_RS23265 0.15 +0.2 783,913 + BPHYT_RS23265 0.22 -1.9 784,068 + BPHYT_RS23265 0.31 -0.4 784,144 + BPHYT_RS23265 0.36 -0.3 784,278 + BPHYT_RS23265 0.44 +1.2 784,433 - BPHYT_RS23265 0.54 +0.2
Or see this region's nucleotide sequence