Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22905 and BPHYT_RS22910 are separated by 24 nucleotides BPHYT_RS22910 and BPHYT_RS22915 are separated by 46 nucleotides BPHYT_RS22915 and BPHYT_RS22920 are separated by 54 nucleotides
BPHYT_RS22905: BPHYT_RS22905 - secretin, at 697,566 to 699,353
_RS22905
BPHYT_RS22910: BPHYT_RS22910 - hypothetical protein, at 699,378 to 699,671
_RS22910
BPHYT_RS22915: BPHYT_RS22915 - pilus assembly protein CpaE, at 699,718 to 700,920
_RS22915
BPHYT_RS22920: BPHYT_RS22920 - pilus assembly protein CpaF, at 700,975 to 702,357
_RS22920
Position (kb) 699
700
701 Strain fitness (log2 ratio) -1
0
1 at 698.769 kb on + strand, within BPHYT_RS22905 at 698.770 kb on - strand, within BPHYT_RS22905 at 699.186 kb on + strand at 699.352 kb on - strand at 699.594 kb on + strand, within BPHYT_RS22910 at 699.715 kb on - strand at 700.180 kb on + strand, within BPHYT_RS22915 at 700.353 kb on - strand, within BPHYT_RS22915 at 701.357 kb on + strand, within BPHYT_RS22920 at 701.357 kb on + strand at 701.358 kb on - strand, within BPHYT_RS22920 at 701.358 kb on - strand, within BPHYT_RS22920 at 701.358 kb on - strand, within BPHYT_RS22920 at 701.360 kb on - strand, within BPHYT_RS22920
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 698,769 + BPHYT_RS22905 0.67 +0.5 698,770 - BPHYT_RS22905 0.67 -0.8 699,186 + +1.2 699,352 - -0.4 699,594 + BPHYT_RS22910 0.73 +1.2 699,715 - +1.6 700,180 + BPHYT_RS22915 0.38 +1.2 700,353 - BPHYT_RS22915 0.53 -0.4 701,357 + BPHYT_RS22920 0.28 +0.6 701,357 + +1.2 701,358 - BPHYT_RS22920 0.28 -0.4 701,358 - BPHYT_RS22920 0.28 +0.0 701,358 - BPHYT_RS22920 0.28 -0.6 701,360 - BPHYT_RS22920 0.28 -0.3
Or see this region's nucleotide sequence