Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22810

Experiment: 24hr PHYTO8 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22800 and BPHYT_RS22805 are separated by 4 nucleotidesBPHYT_RS22805 and BPHYT_RS22810 overlap by 4 nucleotidesBPHYT_RS22810 and BPHYT_RS22815 are separated by 26 nucleotides BPHYT_RS22800: BPHYT_RS22800 - cell envelope biogenesis protein TonB, at 675,513 to 676,196 _RS22800 BPHYT_RS22805: BPHYT_RS22805 - biopolymer transporter ExbD, at 676,201 to 676,599 _RS22805 BPHYT_RS22810: BPHYT_RS22810 - biopolymer transporter ExbB, at 676,596 to 677,243 _RS22810 BPHYT_RS22815: BPHYT_RS22815 - TonB-denpendent receptor, at 677,270 to 679,615 _RS22815 Position (kb) 676 677 678Strain fitness (log2 ratio) -2 -1 0 1at 675.600 kb on + strand, within BPHYT_RS22800at 677.006 kb on + strand, within BPHYT_RS22810at 677.006 kb on + strand, within BPHYT_RS22810at 677.438 kb on + strandat 677.616 kb on + strand, within BPHYT_RS22815at 677.763 kb on + strand, within BPHYT_RS22815at 678.099 kb on + strand, within BPHYT_RS22815

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1
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675,600 + BPHYT_RS22800 0.13 -1.9
677,006 + BPHYT_RS22810 0.63 -2.2
677,006 + BPHYT_RS22810 0.63 -1.1
677,438 + +0.5
677,616 + BPHYT_RS22815 0.15 -0.5
677,763 + BPHYT_RS22815 0.21 -1.1
678,099 + BPHYT_RS22815 0.35 -1.4

Or see this region's nucleotide sequence