Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21855 and BPHYT_RS21860 are separated by 778 nucleotides BPHYT_RS21860 and BPHYT_RS21865 are separated by 350 nucleotides
BPHYT_RS21855: BPHYT_RS21855 - hypothetical protein, at 456,949 to 457,716
_RS21855
BPHYT_RS21860: BPHYT_RS21860 - hypothetical protein, at 458,495 to 458,953
_RS21860
BPHYT_RS21865: BPHYT_RS21865 - chemotaxis protein, at 459,304 to 459,729
_RS21865
Position (kb) 458
459 Strain fitness (log2 ratio) -2
-1
0
1
2 at 457.559 kb on + strand, within BPHYT_RS21855 at 457.560 kb on - strand, within BPHYT_RS21855 at 457.560 kb on - strand at 457.560 kb on - strand, within BPHYT_RS21855 at 457.787 kb on + strand at 457.788 kb on - strand at 457.879 kb on - strand at 457.914 kb on + strand at 458.069 kb on + strand at 458.074 kb on - strand at 458.199 kb on - strand at 458.203 kb on + strand at 458.203 kb on + strand at 458.204 kb on - strand at 458.323 kb on + strand at 458.324 kb on - strand at 458.444 kb on + strand at 458.445 kb on - strand at 458.497 kb on - strand at 458.653 kb on - strand, within BPHYT_RS21860 at 459.012 kb on + strand at 459.059 kb on - strand at 459.253 kb on - strand at 459.278 kb on + strand at 459.699 kb on + strand at 459.800 kb on - strand at 459.871 kb on - strand at 459.881 kb on - strand at 459.929 kb on + strand at 459.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 457,559 + BPHYT_RS21855 0.79 +0.2 457,560 - BPHYT_RS21855 0.80 +1.6 457,560 - +2.2 457,560 - BPHYT_RS21855 0.80 -0.9 457,787 + -0.4 457,788 - -0.2 457,879 - +0.9 457,914 + +0.8 458,069 + -2.1 458,074 - -0.5 458,199 - -0.4 458,203 + -0.9 458,203 + -0.4 458,204 - +0.7 458,323 + -1.7 458,324 - -0.0 458,444 + -0.7 458,445 - +0.3 458,497 - -1.4 458,653 - BPHYT_RS21860 0.34 -1.1 459,012 + +1.0 459,059 - -0.5 459,253 - -0.6 459,278 + -0.1 459,699 + +0.6 459,800 - -0.2 459,871 - +0.0 459,881 - -0.8 459,929 + -0.5 459,930 - +0.0
Or see this region's nucleotide sequence