Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21590 and BPHYT_RS21595 are separated by 161 nucleotides BPHYT_RS21595 and BPHYT_RS21600 are separated by 183 nucleotides BPHYT_RS21600 and BPHYT_RS21605 are separated by 106 nucleotides
BPHYT_RS21590: BPHYT_RS21590 - NmrA family transcriptional regulator, at 400,778 to 401,656
_RS21590
BPHYT_RS21595: BPHYT_RS21595 - TetR family transcriptional regulator, at 401,818 to 402,390
_RS21595
BPHYT_RS21600: BPHYT_RS21600 - 3-oxoacyl-ACP reductase, at 402,574 to 403,284
_RS21600
BPHYT_RS21605: BPHYT_RS21605 - NmrA family transcriptional regulator, at 403,391 to 404,143
_RS21605
Position (kb) 402
403
404 Strain fitness (log2 ratio) -1
0
1 at 401.784 kb on - strand at 401.791 kb on + strand at 402.110 kb on - strand, within BPHYT_RS21595 at 402.173 kb on - strand, within BPHYT_RS21595 at 402.461 kb on + strand at 402.522 kb on + strand at 402.792 kb on - strand, within BPHYT_RS21600 at 403.034 kb on + strand, within BPHYT_RS21600 at 403.035 kb on - strand, within BPHYT_RS21600 at 403.035 kb on - strand, within BPHYT_RS21600 at 403.035 kb on - strand, within BPHYT_RS21600 at 403.197 kb on - strand, within BPHYT_RS21600 at 403.290 kb on - strand at 403.290 kb on - strand at 403.349 kb on + strand at 403.350 kb on - strand at 403.380 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 401,784 - -0.8 401,791 + +0.4 402,110 - BPHYT_RS21595 0.51 +0.2 402,173 - BPHYT_RS21595 0.62 -0.6 402,461 + -0.2 402,522 + +0.2 402,792 - BPHYT_RS21600 0.31 -0.9 403,034 + BPHYT_RS21600 0.65 -0.8 403,035 - BPHYT_RS21600 0.65 +0.4 403,035 - BPHYT_RS21600 0.65 -0.2 403,035 - BPHYT_RS21600 0.65 +0.6 403,197 - BPHYT_RS21600 0.88 +0.4 403,290 - -0.4 403,290 - +0.2 403,349 + -0.4 403,350 - -0.0 403,380 + +0.6
Or see this region's nucleotide sequence