Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09290

Experiment: 24hr PHYTO8 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09285 and BPHYT_RS09290 are separated by 154 nucleotidesBPHYT_RS09290 and BPHYT_RS09295 are separated by 121 nucleotidesBPHYT_RS09295 and BPHYT_RS09300 are separated by 46 nucleotides BPHYT_RS09285: BPHYT_RS09285 - acetylornithine deacetylase, at 2,103,355 to 2,104,569 _RS09285 BPHYT_RS09290: BPHYT_RS09290 - helicase, at 2,104,724 to 2,108,206 _RS09290 BPHYT_RS09295: BPHYT_RS09295 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,108,328 to 2,109,044 _RS09295 BPHYT_RS09300: BPHYT_RS09300 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,109,091 to 2,109,570 _RS09300 Position (kb) 2104 2105 2106 2107 2108 2109Strain fitness (log2 ratio) -1 0 1 2at 2103.787 kb on - strand, within BPHYT_RS09285at 2103.845 kb on + strand, within BPHYT_RS09285at 2103.846 kb on - strand, within BPHYT_RS09285at 2103.899 kb on + strandat 2103.899 kb on + strand, within BPHYT_RS09285at 2103.900 kb on - strand, within BPHYT_RS09285at 2103.900 kb on - strand, within BPHYT_RS09285at 2104.236 kb on - strand, within BPHYT_RS09285at 2104.237 kb on + strand, within BPHYT_RS09285at 2104.237 kb on + strand, within BPHYT_RS09285at 2104.603 kb on - strandat 2104.720 kb on - strandat 2105.099 kb on - strand, within BPHYT_RS09290at 2105.361 kb on + strand, within BPHYT_RS09290at 2105.362 kb on - strand, within BPHYT_RS09290at 2105.600 kb on + strand, within BPHYT_RS09290at 2105.600 kb on + strand, within BPHYT_RS09290at 2105.600 kb on + strand, within BPHYT_RS09290at 2105.601 kb on - strand, within BPHYT_RS09290at 2105.679 kb on - strand, within BPHYT_RS09290at 2106.423 kb on + strand, within BPHYT_RS09290at 2106.424 kb on - strand, within BPHYT_RS09290at 2106.478 kb on - strand, within BPHYT_RS09290at 2106.634 kb on - strand, within BPHYT_RS09290at 2106.712 kb on + strand, within BPHYT_RS09290at 2106.714 kb on + strand, within BPHYT_RS09290at 2106.714 kb on + strand, within BPHYT_RS09290at 2106.714 kb on + strand, within BPHYT_RS09290at 2106.846 kb on + strand, within BPHYT_RS09290at 2107.294 kb on + strand, within BPHYT_RS09290at 2107.398 kb on + strand, within BPHYT_RS09290at 2107.693 kb on - strand, within BPHYT_RS09290at 2107.801 kb on - strand, within BPHYT_RS09290at 2108.178 kb on + strandat 2108.206 kb on + strandat 2108.252 kb on - strandat 2108.282 kb on + strandat 2108.283 kb on - strandat 2108.288 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1
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2,103,787 - BPHYT_RS09285 0.36 +0.2
2,103,845 + BPHYT_RS09285 0.40 -0.5
2,103,846 - BPHYT_RS09285 0.40 +2.3
2,103,899 + +1.0
2,103,899 + BPHYT_RS09285 0.45 -0.0
2,103,900 - BPHYT_RS09285 0.45 +0.5
2,103,900 - BPHYT_RS09285 0.45 +0.3
2,104,236 - BPHYT_RS09285 0.73 -0.2
2,104,237 + BPHYT_RS09285 0.73 -0.4
2,104,237 + BPHYT_RS09285 0.73 +0.0
2,104,603 - +0.2
2,104,720 - -0.9
2,105,099 - BPHYT_RS09290 0.11 +0.7
2,105,361 + BPHYT_RS09290 0.18 +1.6
2,105,362 - BPHYT_RS09290 0.18 -0.9
2,105,600 + BPHYT_RS09290 0.25 -0.4
2,105,600 + BPHYT_RS09290 0.25 -1.5
2,105,600 + BPHYT_RS09290 0.25 -0.3
2,105,601 - BPHYT_RS09290 0.25 -0.1
2,105,679 - BPHYT_RS09290 0.27 +0.3
2,106,423 + BPHYT_RS09290 0.49 +0.8
2,106,424 - BPHYT_RS09290 0.49 +1.7
2,106,478 - BPHYT_RS09290 0.50 -0.3
2,106,634 - BPHYT_RS09290 0.55 +0.3
2,106,712 + BPHYT_RS09290 0.57 -0.3
2,106,714 + BPHYT_RS09290 0.57 +1.3
2,106,714 + BPHYT_RS09290 0.57 -0.1
2,106,714 + BPHYT_RS09290 0.57 +0.9
2,106,846 + BPHYT_RS09290 0.61 -1.0
2,107,294 + BPHYT_RS09290 0.74 +2.3
2,107,398 + BPHYT_RS09290 0.77 -0.1
2,107,693 - BPHYT_RS09290 0.85 -0.9
2,107,801 - BPHYT_RS09290 0.88 +0.5
2,108,178 + +2.3
2,108,206 + +0.3
2,108,252 - -0.5
2,108,282 + +0.9
2,108,283 - -0.5
2,108,288 - +0.3

Or see this region's nucleotide sequence