Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09000 and BPHYT_RS09005 are separated by 322 nucleotides BPHYT_RS09005 and BPHYT_RS09010 overlap by 49 nucleotides BPHYT_RS09010 and BPHYT_RS09015 are separated by 330 nucleotides
BPHYT_RS09000: BPHYT_RS09000 - competence protein ComE, at 2,037,370 to 2,037,960
_RS09000
BPHYT_RS09005: BPHYT_RS09005 - MerR family transcriptional regulator, at 2,038,283 to 2,038,777
_RS09005
BPHYT_RS09010: BPHYT_RS09010 - 4-carboxymuconolactone decarboxylase, at 2,038,729 to 2,039,187
_RS09010
BPHYT_RS09015: BPHYT_RS09015 - multidrug transporter MatE, at 2,039,518 to 2,040,897
_RS09015
Position (kb)
2038
2039 Strain fitness (log2 ratio)
-1
0
1
2 at 2037.303 kb on + strand at 2037.304 kb on - strand at 2037.365 kb on + strand at 2037.610 kb on - strand, within BPHYT_RS09000 at 2037.943 kb on + strand at 2037.943 kb on + strand at 2037.944 kb on - strand at 2037.944 kb on - strand at 2037.944 kb on - strand at 2037.944 kb on - strand at 2038.055 kb on - strand at 2038.060 kb on + strand at 2038.061 kb on - strand at 2038.166 kb on - strand at 2038.203 kb on + strand at 2038.248 kb on + strand at 2038.291 kb on + strand at 2038.348 kb on + strand, within BPHYT_RS09005 at 2038.348 kb on + strand, within BPHYT_RS09005 at 2038.349 kb on - strand, within BPHYT_RS09005 at 2038.361 kb on + strand, within BPHYT_RS09005 at 2038.418 kb on + strand, within BPHYT_RS09005 at 2038.634 kb on + strand, within BPHYT_RS09005 at 2038.637 kb on + strand, within BPHYT_RS09005 at 2038.638 kb on - strand, within BPHYT_RS09005 at 2038.638 kb on - strand, within BPHYT_RS09005 at 2038.850 kb on + strand, within BPHYT_RS09010 at 2039.044 kb on + strand, within BPHYT_RS09010 at 2039.044 kb on + strand, within BPHYT_RS09010 at 2039.321 kb on - strand at 2039.321 kb on - strand at 2039.324 kb on - strand at 2039.359 kb on + strand at 2039.360 kb on - strand at 2039.562 kb on - strand at 2039.699 kb on + strand, within BPHYT_RS09015 at 2039.699 kb on + strand, within BPHYT_RS09015 at 2039.700 kb on - strand, within BPHYT_RS09015 at 2039.768 kb on + strand, within BPHYT_RS09015
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 2,037,303 + +0.5 2,037,304 - -0.4 2,037,365 + -0.1 2,037,610 - BPHYT_RS09000 0.41 -0.7 2,037,943 + +0.2 2,037,943 + +0.6 2,037,944 - -1.4 2,037,944 - +0.6 2,037,944 - -0.3 2,037,944 - -0.1 2,038,055 - -0.7 2,038,060 + +0.3 2,038,061 - -0.4 2,038,166 - +0.8 2,038,203 + +1.5 2,038,248 + -0.3 2,038,291 + +0.2 2,038,348 + BPHYT_RS09005 0.13 +0.8 2,038,348 + BPHYT_RS09005 0.13 -1.2 2,038,349 - BPHYT_RS09005 0.13 +1.5 2,038,361 + BPHYT_RS09005 0.16 +1.9 2,038,418 + BPHYT_RS09005 0.27 -0.0 2,038,634 + BPHYT_RS09005 0.71 +0.9 2,038,637 + BPHYT_RS09005 0.72 -0.1 2,038,638 - BPHYT_RS09005 0.72 -0.1 2,038,638 - BPHYT_RS09005 0.72 +0.5 2,038,850 + BPHYT_RS09010 0.26 +0.1 2,039,044 + BPHYT_RS09010 0.69 -0.4 2,039,044 + BPHYT_RS09010 0.69 -0.8 2,039,321 - +0.2 2,039,321 - +1.2 2,039,324 - +0.4 2,039,359 + +0.2 2,039,360 - +0.9 2,039,562 - +1.2 2,039,699 + BPHYT_RS09015 0.13 -0.6 2,039,699 + BPHYT_RS09015 0.13 +0.8 2,039,700 - BPHYT_RS09015 0.13 -0.3 2,039,768 + BPHYT_RS09015 0.18 +0.1
Or see this region's nucleotide sequence