Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03825 and BPHYT_RS03830 are separated by 92 nucleotides BPHYT_RS03830 and BPHYT_RS03835 overlap by 4 nucleotides
BPHYT_RS03825: BPHYT_RS03825 - enoyl-CoA hydratase, at 864,289 to 865,113
_RS03825
BPHYT_RS03830: BPHYT_RS03830 - hypothetical protein, at 865,206 to 865,820
_RS03830
BPHYT_RS03835: BPHYT_RS03835 - D-lactate dehydrogenase, at 865,817 to 866,815
_RS03835
Position (kb) 865
866 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 864.206 kb on - strand at 864.286 kb on + strand at 864.287 kb on - strand at 864.287 kb on - strand at 864.389 kb on + strand at 865.109 kb on - strand at 865.109 kb on - strand at 865.126 kb on - strand at 865.126 kb on - strand at 865.151 kb on - strand at 865.185 kb on + strand at 865.185 kb on + strand at 865.186 kb on - strand at 865.190 kb on - strand at 865.190 kb on - strand at 865.215 kb on - strand at 865.215 kb on - strand at 865.229 kb on + strand at 865.234 kb on + strand at 865.247 kb on - strand at 865.371 kb on + strand, within BPHYT_RS03830 at 865.483 kb on - strand, within BPHYT_RS03830 at 865.483 kb on - strand, within BPHYT_RS03830 at 865.721 kb on - strand, within BPHYT_RS03830 at 866.034 kb on + strand, within BPHYT_RS03835 at 866.035 kb on - strand, within BPHYT_RS03835 at 866.103 kb on - strand, within BPHYT_RS03835 at 866.178 kb on + strand, within BPHYT_RS03835 at 866.299 kb on - strand, within BPHYT_RS03835 at 866.299 kb on - strand, within BPHYT_RS03835 at 866.437 kb on + strand, within BPHYT_RS03835 at 866.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 864,206 - -0.5 864,286 + +0.3 864,287 - -0.3 864,287 - +0.5 864,389 + +1.2 865,109 - +0.8 865,109 - -0.1 865,126 - -0.1 865,126 - -0.5 865,151 - +0.2 865,185 + +0.1 865,185 + -0.8 865,186 - -1.3 865,190 - +1.4 865,190 - +1.0 865,215 - -1.6 865,215 - -0.3 865,229 + +0.1 865,234 + +0.3 865,247 - +2.2 865,371 + BPHYT_RS03830 0.27 -0.4 865,483 - BPHYT_RS03830 0.45 +0.5 865,483 - BPHYT_RS03830 0.45 +0.5 865,721 - BPHYT_RS03830 0.84 +1.2 866,034 + BPHYT_RS03835 0.22 -0.4 866,035 - BPHYT_RS03835 0.22 -2.9 866,103 - BPHYT_RS03835 0.29 -1.2 866,178 + BPHYT_RS03835 0.36 -0.3 866,299 - BPHYT_RS03835 0.48 +0.4 866,299 - BPHYT_RS03835 0.48 -0.4 866,437 + BPHYT_RS03835 0.62 -1.3 866,743 - -0.0
Or see this region's nucleotide sequence