Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02360 and BPHYT_RS02365 are separated by 71 nucleotides BPHYT_RS02365 and BPHYT_RS02370 are separated by 53 nucleotides BPHYT_RS02370 and BPHYT_RS02375 overlap by 4 nucleotides BPHYT_RS02375 and BPHYT_RS02380 are separated by 105 nucleotides
BPHYT_RS02360: BPHYT_RS02360 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 541,189 to 541,671
_RS02360
BPHYT_RS02365: BPHYT_RS02365 - hypothetical protein, at 541,743 to 541,982
_RS02365
BPHYT_RS02370: BPHYT_RS02370 - type III pantothenate kinase, at 542,036 to 542,848
_RS02370
BPHYT_RS02375: BPHYT_RS02375 - biotin--protein ligase, at 542,845 to 543,738
_RS02375
BPHYT_RS02380: BPHYT_RS02380 - ABC transporter permease, at 543,844 to 544,977
_RS02380
Position (kb) 542
543 Strain fitness (log2 ratio) -2
-1
0
1 at 541.151 kb on + strand at 541.151 kb on + strand at 541.151 kb on + strand at 541.659 kb on + strand at 541.659 kb on + strand at 541.721 kb on - strand at 541.721 kb on - strand at 541.744 kb on + strand at 541.745 kb on - strand at 541.944 kb on - strand, within BPHYT_RS02365 at 541.959 kb on + strand at 541.960 kb on - strand at 541.960 kb on - strand at 543.750 kb on + strand at 543.750 kb on + strand at 543.805 kb on + strand at 543.806 kb on - strand at 543.806 kb on - strand at 543.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 541,151 + +0.5 541,151 + -1.4 541,151 + -0.1 541,659 + -1.2 541,659 + +0.0 541,721 - +0.1 541,721 - -0.8 541,744 + +1.4 541,745 - -0.1 541,944 - BPHYT_RS02365 0.84 -1.9 541,959 + +0.1 541,960 - +1.7 541,960 - -0.8 543,750 + -1.5 543,750 + +0.6 543,805 + -0.6 543,806 - -0.7 543,806 - -1.4 543,817 + -0.3
Or see this region's nucleotide sequence