Experiment: 24hr PHYTO8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00270 and BPHYT_RS00275 are separated by 37 nucleotides BPHYT_RS00275 and BPHYT_RS00280 are separated by 2 nucleotides BPHYT_RS00280 and BPHYT_RS00285 are separated by 9 nucleotides BPHYT_RS00285 and BPHYT_RS00290 overlap by 16 nucleotides
BPHYT_RS00270: BPHYT_RS00270 - branched-chain amino acid ABC transporter, at 69,485 to 70,186
_RS00270
BPHYT_RS00275: BPHYT_RS00275 - urease subunit beta, at 70,224 to 70,910
_RS00275
BPHYT_RS00280: BPHYT_RS00280 - urease subunit alpha, at 70,913 to 72,628
_RS00280
BPHYT_RS00285: BPHYT_RS00285 - urease accessory protein UreG, at 72,638 to 73,252
_RS00285
BPHYT_RS00290: BPHYT_RS00290 - hypothetical protein, at 73,237 to 74,064
_RS00290
Position (kb) 70
71
72
73 Strain fitness (log2 ratio) -2
-1
0
1
2 at 70.094 kb on + strand, within BPHYT_RS00270 at 70.095 kb on - strand, within BPHYT_RS00270 at 70.306 kb on + strand, within BPHYT_RS00275 at 70.307 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.460 kb on - strand, within BPHYT_RS00275 at 70.607 kb on + strand, within BPHYT_RS00275 at 70.608 kb on - strand, within BPHYT_RS00275 at 70.683 kb on - strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.772 kb on + strand, within BPHYT_RS00275 at 70.773 kb on - strand, within BPHYT_RS00275 at 70.908 kb on + strand at 70.908 kb on + strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.909 kb on - strand at 70.941 kb on - strand at 71.200 kb on + strand, within BPHYT_RS00280 at 71.200 kb on + strand, within BPHYT_RS00280 at 71.733 kb on - strand, within BPHYT_RS00280 at 71.733 kb on - strand, within BPHYT_RS00280 at 71.842 kb on + strand, within BPHYT_RS00280 at 71.842 kb on + strand, within BPHYT_RS00280 at 71.843 kb on - strand at 72.062 kb on - strand, within BPHYT_RS00280 at 72.062 kb on - strand, within BPHYT_RS00280 at 72.062 kb on - strand, within BPHYT_RS00280 at 72.131 kb on + strand, within BPHYT_RS00280 at 72.134 kb on - strand, within BPHYT_RS00280 at 72.143 kb on + strand at 72.144 kb on - strand, within BPHYT_RS00280 at 72.614 kb on + strand at 72.615 kb on - strand at 72.615 kb on - strand at 72.615 kb on - strand at 72.626 kb on + strand at 72.626 kb on + strand at 72.627 kb on - strand at 72.649 kb on - strand at 73.024 kb on + strand, within BPHYT_RS00285 at 73.024 kb on + strand, within BPHYT_RS00285 at 73.025 kb on - strand, within BPHYT_RS00285 at 73.097 kb on + strand, within BPHYT_RS00285 at 73.097 kb on + strand, within BPHYT_RS00285 at 73.097 kb on + strand, within BPHYT_RS00285 at 73.098 kb on - strand, within BPHYT_RS00285 at 73.098 kb on - strand, within BPHYT_RS00285 at 73.135 kb on - strand, within BPHYT_RS00285 at 73.240 kb on + strand at 73.241 kb on - strand at 73.384 kb on + strand, within BPHYT_RS00290 at 73.385 kb on - strand, within BPHYT_RS00290 at 73.463 kb on + strand, within BPHYT_RS00290 at 73.464 kb on - strand, within BPHYT_RS00290
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO8 MOI 1 remove 70,094 + BPHYT_RS00270 0.87 -1.0 70,095 - BPHYT_RS00270 0.87 -0.4 70,306 + BPHYT_RS00275 0.12 -0.5 70,307 - BPHYT_RS00275 0.12 -0.3 70,460 - BPHYT_RS00275 0.34 -0.1 70,460 - BPHYT_RS00275 0.34 +0.6 70,460 - BPHYT_RS00275 0.34 -0.1 70,607 + BPHYT_RS00275 0.56 -0.5 70,608 - BPHYT_RS00275 0.56 +0.3 70,683 - BPHYT_RS00275 0.67 -0.4 70,772 + BPHYT_RS00275 0.80 -0.6 70,772 + BPHYT_RS00275 0.80 +0.0 70,772 + BPHYT_RS00275 0.80 +0.0 70,773 - BPHYT_RS00275 0.80 -0.5 70,908 + -2.6 70,908 + +0.4 70,909 - +0.3 70,909 - +1.0 70,909 - -0.6 70,909 - +0.1 70,941 - -2.1 71,200 + BPHYT_RS00280 0.17 -2.1 71,200 + BPHYT_RS00280 0.17 +1.6 71,733 - BPHYT_RS00280 0.48 +1.2 71,733 - BPHYT_RS00280 0.48 -0.6 71,842 + BPHYT_RS00280 0.54 +0.6 71,842 + BPHYT_RS00280 0.54 -2.4 71,843 - -0.6 72,062 - BPHYT_RS00280 0.67 +1.2 72,062 - BPHYT_RS00280 0.67 +0.3 72,062 - BPHYT_RS00280 0.67 +1.5 72,131 + BPHYT_RS00280 0.71 -0.8 72,134 - BPHYT_RS00280 0.71 +1.6 72,143 + +0.6 72,144 - BPHYT_RS00280 0.72 +0.3 72,614 + -0.7 72,615 - -0.7 72,615 - +2.2 72,615 - -0.0 72,626 + -0.2 72,626 + +0.1 72,627 - +0.9 72,649 - -0.4 73,024 + BPHYT_RS00285 0.63 +0.3 73,024 + BPHYT_RS00285 0.63 +1.0 73,025 - BPHYT_RS00285 0.63 +0.8 73,097 + BPHYT_RS00285 0.75 -0.5 73,097 + BPHYT_RS00285 0.75 -0.5 73,097 + BPHYT_RS00285 0.75 -0.7 73,098 - BPHYT_RS00285 0.75 -0.2 73,098 - BPHYT_RS00285 0.75 +1.4 73,135 - BPHYT_RS00285 0.81 -0.5 73,240 + +0.6 73,241 - -0.2 73,384 + BPHYT_RS00290 0.18 -0.4 73,385 - BPHYT_RS00290 0.18 -0.1 73,463 + BPHYT_RS00290 0.27 -0.8 73,464 - BPHYT_RS00290 0.27 -0.3
Or see this region's nucleotide sequence