Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35225 and BPHYT_RS35230 are separated by 36 nucleotides BPHYT_RS35230 and BPHYT_RS35235 are separated by 183 nucleotides BPHYT_RS35235 and BPHYT_RS35240 are separated by 57 nucleotides BPHYT_RS35240 and BPHYT_RS35245 are separated by 128 nucleotides BPHYT_RS35245 and BPHYT_RS35250 are separated by 55 nucleotides
BPHYT_RS35225: BPHYT_RS35225 - RNA polymerase subunit sigma-24, at 3,506,519 to 3,507,793
_RS35225
BPHYT_RS35230: BPHYT_RS35230 - type 11 methyltransferase, at 3,507,830 to 3,508,519
_RS35230
BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320
_RS35235
BPHYT_RS35240: BPHYT_RS35240 - membrane protein, at 3,509,378 to 3,509,956
_RS35240
BPHYT_RS35245: BPHYT_RS35245 - hypothetical protein, at 3,510,085 to 3,510,261
_RS35245
BPHYT_RS35250: BPHYT_RS35250 - alcohol dehydrogenase, at 3,510,317 to 3,511,282
_RS35250
Position (kb)
3508
3509
3510 Strain fitness (log2 ratio)
-1
0
1
2 at 3507.987 kb on + strand, within BPHYT_RS35230 at 3508.253 kb on - strand, within BPHYT_RS35230 at 3508.253 kb on - strand at 3508.579 kb on + strand at 3508.628 kb on - strand at 3508.698 kb on - strand at 3508.783 kb on - strand, within BPHYT_RS35235 at 3509.141 kb on + strand, within BPHYT_RS35235 at 3509.325 kb on + strand at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.628 kb on + strand, within BPHYT_RS35240 at 3509.730 kb on + strand, within BPHYT_RS35240 at 3509.784 kb on + strand, within BPHYT_RS35240 at 3509.785 kb on - strand, within BPHYT_RS35240 at 3509.944 kb on - strand at 3510.087 kb on - strand at 3510.129 kb on + strand, within BPHYT_RS35245 at 3510.129 kb on + strand, within BPHYT_RS35245 at 3510.210 kb on - strand, within BPHYT_RS35245 at 3510.270 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 3,507,987 + BPHYT_RS35230 0.23 +0.8 3,508,253 - BPHYT_RS35230 0.61 +0.6 3,508,253 - +2.2 3,508,579 + +0.8 3,508,628 - +0.3 3,508,698 - -1.5 3,508,783 - BPHYT_RS35235 0.13 -1.4 3,509,141 + BPHYT_RS35235 0.71 -0.7 3,509,325 + -1.1 3,509,628 + BPHYT_RS35240 0.43 +0.1 3,509,628 + BPHYT_RS35240 0.43 -0.4 3,509,628 + BPHYT_RS35240 0.43 +0.2 3,509,628 + BPHYT_RS35240 0.43 -0.2 3,509,730 + BPHYT_RS35240 0.61 +0.1 3,509,784 + BPHYT_RS35240 0.70 -1.0 3,509,785 - BPHYT_RS35240 0.70 -0.2 3,509,944 - -0.9 3,510,087 - -1.6 3,510,129 + BPHYT_RS35245 0.25 -0.3 3,510,129 + BPHYT_RS35245 0.25 -0.8 3,510,210 - BPHYT_RS35245 0.71 +0.6 3,510,270 + -0.8
Or see this region's nucleotide sequence