Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32695 and BPHYT_RS32700 are separated by 329 nucleotides BPHYT_RS32700 and BPHYT_RS32705 are separated by 160 nucleotides BPHYT_RS32705 and BPHYT_RS32710 overlap by 4 nucleotides
BPHYT_RS32695: BPHYT_RS32695 - prolyl 4-hydroxylase, at 2,911,643 to 2,912,446
_RS32695
BPHYT_RS32700: BPHYT_RS32700 - membrane protein, at 2,912,776 to 2,914,692
_RS32700
BPHYT_RS32705: BPHYT_RS32705 - PAAR motif family protein, at 2,914,853 to 2,914,963
_RS32705
BPHYT_RS32710: BPHYT_RS32710 - hypothetical protein, at 2,914,960 to 2,915,745
_RS32710
Position (kb)
2912
2913
2914
2915 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2911.931 kb on + strand, within BPHYT_RS32695 at 2911.937 kb on + strand, within BPHYT_RS32695 at 2911.938 kb on - strand, within BPHYT_RS32695 at 2912.071 kb on + strand, within BPHYT_RS32695 at 2912.116 kb on - strand, within BPHYT_RS32695 at 2912.141 kb on + strand, within BPHYT_RS32695 at 2912.165 kb on + strand, within BPHYT_RS32695 at 2912.166 kb on - strand, within BPHYT_RS32695 at 2912.414 kb on - strand at 2912.499 kb on + strand at 2912.500 kb on - strand at 2912.500 kb on - strand at 2912.753 kb on - strand at 2913.019 kb on + strand, within BPHYT_RS32700 at 2913.020 kb on - strand, within BPHYT_RS32700 at 2913.020 kb on - strand, within BPHYT_RS32700 at 2913.551 kb on + strand, within BPHYT_RS32700 at 2913.551 kb on + strand, within BPHYT_RS32700 at 2913.552 kb on - strand, within BPHYT_RS32700 at 2913.552 kb on - strand, within BPHYT_RS32700 at 2913.552 kb on - strand, within BPHYT_RS32700 at 2913.777 kb on + strand, within BPHYT_RS32700 at 2913.778 kb on - strand, within BPHYT_RS32700 at 2913.878 kb on + strand, within BPHYT_RS32700 at 2913.955 kb on + strand, within BPHYT_RS32700 at 2913.956 kb on - strand, within BPHYT_RS32700 at 2913.958 kb on + strand, within BPHYT_RS32700 at 2914.347 kb on - strand, within BPHYT_RS32700 at 2914.419 kb on + strand, within BPHYT_RS32700 at 2914.690 kb on + strand at 2914.716 kb on - strand at 2914.804 kb on - strand at 2914.827 kb on + strand at 2914.827 kb on + strand at 2914.827 kb on + strand at 2915.076 kb on + strand, within BPHYT_RS32710 at 2915.127 kb on - strand, within BPHYT_RS32710 at 2915.159 kb on - strand, within BPHYT_RS32710 at 2915.373 kb on + strand, within BPHYT_RS32710 at 2915.510 kb on - strand, within BPHYT_RS32710 at 2915.675 kb on - strand at 2915.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 2,911,931 + BPHYT_RS32695 0.36 +1.0 2,911,937 + BPHYT_RS32695 0.37 +0.3 2,911,938 - BPHYT_RS32695 0.37 +0.9 2,912,071 + BPHYT_RS32695 0.53 +0.3 2,912,116 - BPHYT_RS32695 0.59 -2.4 2,912,141 + BPHYT_RS32695 0.62 +0.3 2,912,165 + BPHYT_RS32695 0.65 +0.1 2,912,166 - BPHYT_RS32695 0.65 +0.2 2,912,414 - -0.5 2,912,499 + +2.4 2,912,500 - -0.4 2,912,500 - +1.1 2,912,753 - +0.3 2,913,019 + BPHYT_RS32700 0.13 -1.3 2,913,020 - BPHYT_RS32700 0.13 +0.2 2,913,020 - BPHYT_RS32700 0.13 -0.2 2,913,551 + BPHYT_RS32700 0.40 -2.2 2,913,551 + BPHYT_RS32700 0.40 -0.4 2,913,552 - BPHYT_RS32700 0.40 -0.3 2,913,552 - BPHYT_RS32700 0.40 +1.1 2,913,552 - BPHYT_RS32700 0.40 +2.0 2,913,777 + BPHYT_RS32700 0.52 -1.1 2,913,778 - BPHYT_RS32700 0.52 -1.0 2,913,878 + BPHYT_RS32700 0.57 -1.6 2,913,955 + BPHYT_RS32700 0.62 +0.5 2,913,956 - BPHYT_RS32700 0.62 +0.4 2,913,958 + BPHYT_RS32700 0.62 -1.0 2,914,347 - BPHYT_RS32700 0.82 -1.2 2,914,419 + BPHYT_RS32700 0.86 -1.1 2,914,690 + -1.8 2,914,716 - -2.0 2,914,804 - -0.0 2,914,827 + +1.1 2,914,827 + +0.3 2,914,827 + -1.8 2,915,076 + BPHYT_RS32710 0.15 -0.5 2,915,127 - BPHYT_RS32710 0.21 -3.0 2,915,159 - BPHYT_RS32710 0.25 -0.2 2,915,373 + BPHYT_RS32710 0.53 -1.2 2,915,510 - BPHYT_RS32710 0.70 +0.4 2,915,675 - +0.8 2,915,675 - +0.3
Or see this region's nucleotide sequence