Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32250 and BPHYT_RS32255 are separated by 459 nucleotides BPHYT_RS32255 and BPHYT_RS32260 are separated by 79 nucleotides BPHYT_RS32260 and BPHYT_RS32265 overlap by 12 nucleotides
BPHYT_RS32250: BPHYT_RS32250 - membrane protein, at 2,805,165 to 2,806,709
_RS32250
BPHYT_RS32255: BPHYT_RS32255 - hypothetical protein, at 2,807,169 to 2,807,399
_RS32255
BPHYT_RS32260: BPHYT_RS32260 - hypothetical protein, at 2,807,479 to 2,807,787
_RS32260
BPHYT_RS32265: BPHYT_RS32265 - transposase, at 2,807,776 to 2,809,371
_RS32265
Position (kb)
2807
2808 Strain fitness (log2 ratio)
-2
-1
0
1 at 2806.705 kb on + strand at 2806.706 kb on - strand at 2806.956 kb on + strand at 2807.009 kb on - strand at 2807.017 kb on - strand at 2807.524 kb on + strand, within BPHYT_RS32260 at 2807.873 kb on - strand at 2807.876 kb on + strand at 2808.054 kb on - strand, within BPHYT_RS32265 at 2808.086 kb on + strand, within BPHYT_RS32265 at 2808.087 kb on - strand, within BPHYT_RS32265 at 2808.199 kb on - strand, within BPHYT_RS32265 at 2808.365 kb on + strand, within BPHYT_RS32265 at 2808.518 kb on + strand, within BPHYT_RS32265 at 2808.519 kb on - strand, within BPHYT_RS32265 at 2808.553 kb on - strand, within BPHYT_RS32265
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 2,806,705 + -0.7 2,806,706 - +1.1 2,806,956 + -1.3 2,807,009 - -1.3 2,807,017 - +0.7 2,807,524 + BPHYT_RS32260 0.15 -2.0 2,807,873 - +1.3 2,807,876 + +1.3 2,808,054 - BPHYT_RS32265 0.17 +0.1 2,808,086 + BPHYT_RS32265 0.19 +0.8 2,808,087 - BPHYT_RS32265 0.19 +0.1 2,808,199 - BPHYT_RS32265 0.27 -0.1 2,808,365 + BPHYT_RS32265 0.37 -0.7 2,808,518 + BPHYT_RS32265 0.46 -0.2 2,808,519 - BPHYT_RS32265 0.47 -2.4 2,808,553 - BPHYT_RS32265 0.49 -0.3
Or see this region's nucleotide sequence