Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31170 and BPHYT_RS31175 are separated by 145 nucleotides
BPHYT_RS31170: BPHYT_RS31170 - hypothetical protein, at 2,550,548 to 2,552,365
_RS31170
BPHYT_RS31175: BPHYT_RS31175 - RNA polymerase sigma70 factor, at 2,552,511 to 2,553,785
_RS31175
Position (kb)
2550
2551
2552
2553 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2549.611 kb on - strand at 2549.691 kb on - strand at 2550.196 kb on - strand at 2551.049 kb on + strand, within BPHYT_RS31170 at 2551.049 kb on + strand, within BPHYT_RS31170 at 2551.049 kb on + strand, within BPHYT_RS31170 at 2551.235 kb on + strand, within BPHYT_RS31170 at 2551.235 kb on + strand, within BPHYT_RS31170 at 2551.236 kb on - strand, within BPHYT_RS31170 at 2551.238 kb on - strand, within BPHYT_RS31170 at 2551.537 kb on - strand, within BPHYT_RS31170 at 2551.625 kb on - strand, within BPHYT_RS31170 at 2551.762 kb on + strand, within BPHYT_RS31170 at 2551.763 kb on - strand, within BPHYT_RS31170 at 2551.770 kb on + strand, within BPHYT_RS31170 at 2552.057 kb on - strand, within BPHYT_RS31170 at 2552.453 kb on + strand at 2552.453 kb on + strand at 2552.454 kb on - strand at 2552.454 kb on - strand at 2552.713 kb on + strand, within BPHYT_RS31175 at 2552.871 kb on + strand, within BPHYT_RS31175 at 2552.872 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2553.010 kb on + strand, within BPHYT_RS31175 at 2553.010 kb on + strand, within BPHYT_RS31175 at 2553.011 kb on - strand, within BPHYT_RS31175 at 2553.055 kb on + strand, within BPHYT_RS31175 at 2553.055 kb on + strand, within BPHYT_RS31175
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 2,549,611 - -1.1 2,549,691 - -0.3 2,550,196 - +1.6 2,551,049 + BPHYT_RS31170 0.28 +0.3 2,551,049 + BPHYT_RS31170 0.28 +0.3 2,551,049 + BPHYT_RS31170 0.28 +0.7 2,551,235 + BPHYT_RS31170 0.38 -0.0 2,551,235 + BPHYT_RS31170 0.38 -1.2 2,551,236 - BPHYT_RS31170 0.38 -1.4 2,551,238 - BPHYT_RS31170 0.38 +1.7 2,551,537 - BPHYT_RS31170 0.54 +0.1 2,551,625 - BPHYT_RS31170 0.59 +0.0 2,551,762 + BPHYT_RS31170 0.67 -3.8 2,551,763 - BPHYT_RS31170 0.67 +0.6 2,551,770 + BPHYT_RS31170 0.67 -0.5 2,552,057 - BPHYT_RS31170 0.83 +1.0 2,552,453 + -1.5 2,552,453 + -0.5 2,552,454 - -0.3 2,552,454 - -0.1 2,552,713 + BPHYT_RS31175 0.16 +1.1 2,552,871 + BPHYT_RS31175 0.28 +1.0 2,552,872 - BPHYT_RS31175 0.28 -0.6 2,552,982 - BPHYT_RS31175 0.37 -0.4 2,552,982 - BPHYT_RS31175 0.37 +0.3 2,552,982 - BPHYT_RS31175 0.37 +2.0 2,553,010 + BPHYT_RS31175 0.39 +0.4 2,553,010 + BPHYT_RS31175 0.39 -0.5 2,553,011 - BPHYT_RS31175 0.39 -1.1 2,553,055 + BPHYT_RS31175 0.43 -0.5 2,553,055 + BPHYT_RS31175 0.43 +1.6
Or see this region's nucleotide sequence