Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29715 and BPHYT_RS29720 are separated by 124 nucleotides BPHYT_RS29720 and BPHYT_RS29725 are separated by 96 nucleotides
BPHYT_RS29715: BPHYT_RS29715 - acylaldehyde oxidase, at 2,217,907 to 2,220,129
_RS29715
BPHYT_RS29720: BPHYT_RS29720 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,220,254 to 2,220,841
_RS29720
BPHYT_RS29725: BPHYT_RS29725 - aromatic compound catabolic protein, at 2,220,938 to 2,221,486
_RS29725
Position (kb)
2220
2221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2219.308 kb on + strand, within BPHYT_RS29715 at 2219.308 kb on + strand, within BPHYT_RS29715 at 2219.850 kb on - strand, within BPHYT_RS29715 at 2219.871 kb on + strand, within BPHYT_RS29715 at 2219.953 kb on - strand at 2220.003 kb on + strand at 2220.056 kb on - strand at 2220.107 kb on + strand at 2220.239 kb on + strand at 2220.335 kb on - strand, within BPHYT_RS29720 at 2220.659 kb on + strand, within BPHYT_RS29720 at 2220.869 kb on - strand at 2220.892 kb on - strand at 2220.946 kb on + strand at 2220.990 kb on + strand at 2221.108 kb on - strand, within BPHYT_RS29725 at 2221.108 kb on - strand, within BPHYT_RS29725 at 2221.132 kb on + strand, within BPHYT_RS29725 at 2221.530 kb on + strand at 2221.531 kb on - strand at 2221.531 kb on - strand at 2221.564 kb on + strand at 2221.573 kb on + strand at 2221.773 kb on + strand at 2221.818 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 2,219,308 + BPHYT_RS29715 0.63 -0.1 2,219,308 + BPHYT_RS29715 0.63 -1.0 2,219,850 - BPHYT_RS29715 0.87 -0.9 2,219,871 + BPHYT_RS29715 0.88 +1.4 2,219,953 - +0.9 2,220,003 + -0.8 2,220,056 - -0.5 2,220,107 + +0.0 2,220,239 + -1.4 2,220,335 - BPHYT_RS29720 0.14 -1.0 2,220,659 + BPHYT_RS29720 0.69 -3.1 2,220,869 - -0.4 2,220,892 - -1.5 2,220,946 + +0.1 2,220,990 + +0.3 2,221,108 - BPHYT_RS29725 0.31 -0.3 2,221,108 - BPHYT_RS29725 0.31 +3.0 2,221,132 + BPHYT_RS29725 0.35 +1.8 2,221,530 + +0.2 2,221,531 - +1.1 2,221,531 - -0.4 2,221,564 + +2.0 2,221,573 + +1.7 2,221,773 + +0.2 2,221,818 + -0.9
Or see this region's nucleotide sequence