Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29720

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29715 and BPHYT_RS29720 are separated by 124 nucleotidesBPHYT_RS29720 and BPHYT_RS29725 are separated by 96 nucleotides BPHYT_RS29715: BPHYT_RS29715 - acylaldehyde oxidase, at 2,217,907 to 2,220,129 _RS29715 BPHYT_RS29720: BPHYT_RS29720 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,220,254 to 2,220,841 _RS29720 BPHYT_RS29725: BPHYT_RS29725 - aromatic compound catabolic protein, at 2,220,938 to 2,221,486 _RS29725 Position (kb) 2220 2221Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2219.308 kb on + strand, within BPHYT_RS29715at 2219.308 kb on + strand, within BPHYT_RS29715at 2219.850 kb on - strand, within BPHYT_RS29715at 2219.871 kb on + strand, within BPHYT_RS29715at 2219.953 kb on - strandat 2220.003 kb on + strandat 2220.056 kb on - strandat 2220.107 kb on + strandat 2220.239 kb on + strandat 2220.335 kb on - strand, within BPHYT_RS29720at 2220.659 kb on + strand, within BPHYT_RS29720at 2220.869 kb on - strandat 2220.892 kb on - strandat 2220.946 kb on + strandat 2220.990 kb on + strandat 2221.108 kb on - strand, within BPHYT_RS29725at 2221.108 kb on - strand, within BPHYT_RS29725at 2221.132 kb on + strand, within BPHYT_RS29725at 2221.530 kb on + strandat 2221.531 kb on - strandat 2221.531 kb on - strandat 2221.564 kb on + strandat 2221.573 kb on + strandat 2221.773 kb on + strandat 2221.818 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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2,219,308 + BPHYT_RS29715 0.63 -0.1
2,219,308 + BPHYT_RS29715 0.63 -1.0
2,219,850 - BPHYT_RS29715 0.87 -0.9
2,219,871 + BPHYT_RS29715 0.88 +1.4
2,219,953 - +0.9
2,220,003 + -0.8
2,220,056 - -0.5
2,220,107 + +0.0
2,220,239 + -1.4
2,220,335 - BPHYT_RS29720 0.14 -1.0
2,220,659 + BPHYT_RS29720 0.69 -3.1
2,220,869 - -0.4
2,220,892 - -1.5
2,220,946 + +0.1
2,220,990 + +0.3
2,221,108 - BPHYT_RS29725 0.31 -0.3
2,221,108 - BPHYT_RS29725 0.31 +3.0
2,221,132 + BPHYT_RS29725 0.35 +1.8
2,221,530 + +0.2
2,221,531 - +1.1
2,221,531 - -0.4
2,221,564 + +2.0
2,221,573 + +1.7
2,221,773 + +0.2
2,221,818 + -0.9

Or see this region's nucleotide sequence