Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28475 and BPHYT_RS28480 are separated by 195 nucleotides BPHYT_RS28480 and BPHYT_RS28485 are separated by 151 nucleotides
BPHYT_RS28475: BPHYT_RS28475 - porin, at 1,942,358 to 1,943,524
_RS28475
BPHYT_RS28480: BPHYT_RS28480 - hydroxyglutarate oxidase, at 1,943,720 to 1,944,916
_RS28480
BPHYT_RS28485: BPHYT_RS28485 - GntR family transcriptional regulator, at 1,945,068 to 1,945,769
_RS28485
Position (kb)
1943
1944
1945 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1942.762 kb on - strand, within BPHYT_RS28475 at 1942.956 kb on + strand, within BPHYT_RS28475 at 1942.957 kb on - strand, within BPHYT_RS28475 at 1942.999 kb on + strand, within BPHYT_RS28475 at 1943.000 kb on - strand, within BPHYT_RS28475 at 1943.029 kb on - strand, within BPHYT_RS28475 at 1943.029 kb on - strand, within BPHYT_RS28475 at 1943.151 kb on + strand, within BPHYT_RS28475 at 1943.152 kb on - strand, within BPHYT_RS28475 at 1943.214 kb on - strand, within BPHYT_RS28475 at 1943.217 kb on + strand, within BPHYT_RS28475 at 1943.218 kb on - strand, within BPHYT_RS28475 at 1943.408 kb on + strand at 1943.409 kb on - strand at 1943.563 kb on + strand at 1943.564 kb on - strand at 1944.465 kb on - strand, within BPHYT_RS28480 at 1944.598 kb on - strand, within BPHYT_RS28480 at 1945.065 kb on - strand at 1945.084 kb on - strand at 1945.401 kb on - strand, within BPHYT_RS28485 at 1945.805 kb on + strand at 1945.813 kb on + strand at 1945.865 kb on - strand at 1945.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 1,942,762 - BPHYT_RS28475 0.35 -1.5 1,942,956 + BPHYT_RS28475 0.51 -0.5 1,942,957 - BPHYT_RS28475 0.51 -0.4 1,942,999 + BPHYT_RS28475 0.55 -1.4 1,943,000 - BPHYT_RS28475 0.55 +0.7 1,943,029 - BPHYT_RS28475 0.57 -2.3 1,943,029 - BPHYT_RS28475 0.57 -1.0 1,943,151 + BPHYT_RS28475 0.68 +1.1 1,943,152 - BPHYT_RS28475 0.68 +0.3 1,943,214 - BPHYT_RS28475 0.73 +2.1 1,943,217 + BPHYT_RS28475 0.74 +0.1 1,943,218 - BPHYT_RS28475 0.74 +0.5 1,943,408 + +0.4 1,943,409 - +0.8 1,943,563 + +0.8 1,943,564 - -1.5 1,944,465 - BPHYT_RS28480 0.62 -1.3 1,944,598 - BPHYT_RS28480 0.73 -0.8 1,945,065 - -2.1 1,945,084 - +1.8 1,945,401 - BPHYT_RS28485 0.47 -1.6 1,945,805 + +0.6 1,945,813 + -1.9 1,945,865 - -0.5 1,945,865 - -0.7
Or see this region's nucleotide sequence