Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25290

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25285 and BPHYT_RS25290 are separated by 115 nucleotidesBPHYT_RS25290 and BPHYT_RS25295 are separated by 14 nucleotidesBPHYT_RS25295 and BPHYT_RS25300 are separated by 57 nucleotidesBPHYT_RS25300 and BPHYT_RS25305 are separated by 12 nucleotides BPHYT_RS25285: BPHYT_RS25285 - AraC family transcriptional regulator, at 1,249,609 to 1,250,643 _RS25285 BPHYT_RS25290: BPHYT_RS25290 - membrane protein, at 1,250,759 to 1,251,451 _RS25290 BPHYT_RS25295: BPHYT_RS25295 - cobalt transporter, at 1,251,466 to 1,251,675 _RS25295 BPHYT_RS25300: BPHYT_RS25300 - transmembrane nitrile hydratase, at 1,251,733 to 1,252,113 _RS25300 BPHYT_RS25305: BPHYT_RS25305 - cobalt-containing nitrile hydratase subunit alpha, at 1,252,126 to 1,252,749 _RS25305 Position (kb) 1250 1251 1252Strain fitness (log2 ratio) -2 -1 0 1at 1249.973 kb on + strand, within BPHYT_RS25285at 1249.973 kb on + strand, within BPHYT_RS25285at 1250.529 kb on - strand, within BPHYT_RS25285at 1250.552 kb on - strandat 1250.686 kb on - strandat 1251.344 kb on + strand, within BPHYT_RS25290at 1251.345 kb on - strand, within BPHYT_RS25290at 1251.345 kb on - strand, within BPHYT_RS25290at 1251.437 kb on + strandat 1251.521 kb on + strand, within BPHYT_RS25295at 1252.092 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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1,249,973 + BPHYT_RS25285 0.35 +1.2
1,249,973 + BPHYT_RS25285 0.35 -0.7
1,250,529 - BPHYT_RS25285 0.89 -0.4
1,250,552 - +0.2
1,250,686 - +0.4
1,251,344 + BPHYT_RS25290 0.84 -0.9
1,251,345 - BPHYT_RS25290 0.85 -1.7
1,251,345 - BPHYT_RS25290 0.85 -2.2
1,251,437 + +1.1
1,251,521 + BPHYT_RS25295 0.26 -0.6
1,252,092 - -0.0

Or see this region's nucleotide sequence