Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25285 and BPHYT_RS25290 are separated by 115 nucleotides BPHYT_RS25290 and BPHYT_RS25295 are separated by 14 nucleotides BPHYT_RS25295 and BPHYT_RS25300 are separated by 57 nucleotides BPHYT_RS25300 and BPHYT_RS25305 are separated by 12 nucleotides
BPHYT_RS25285: BPHYT_RS25285 - AraC family transcriptional regulator, at 1,249,609 to 1,250,643
_RS25285
BPHYT_RS25290: BPHYT_RS25290 - membrane protein, at 1,250,759 to 1,251,451
_RS25290
BPHYT_RS25295: BPHYT_RS25295 - cobalt transporter, at 1,251,466 to 1,251,675
_RS25295
BPHYT_RS25300: BPHYT_RS25300 - transmembrane nitrile hydratase, at 1,251,733 to 1,252,113
_RS25300
BPHYT_RS25305: BPHYT_RS25305 - cobalt-containing nitrile hydratase subunit alpha, at 1,252,126 to 1,252,749
_RS25305
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-2
-1
0
1 at 1249.973 kb on + strand, within BPHYT_RS25285 at 1249.973 kb on + strand, within BPHYT_RS25285 at 1250.529 kb on - strand, within BPHYT_RS25285 at 1250.552 kb on - strand at 1250.686 kb on - strand at 1251.344 kb on + strand, within BPHYT_RS25290 at 1251.345 kb on - strand, within BPHYT_RS25290 at 1251.345 kb on - strand, within BPHYT_RS25290 at 1251.437 kb on + strand at 1251.521 kb on + strand, within BPHYT_RS25295 at 1252.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 1,249,973 + BPHYT_RS25285 0.35 +1.2 1,249,973 + BPHYT_RS25285 0.35 -0.7 1,250,529 - BPHYT_RS25285 0.89 -0.4 1,250,552 - +0.2 1,250,686 - +0.4 1,251,344 + BPHYT_RS25290 0.84 -0.9 1,251,345 - BPHYT_RS25290 0.85 -1.7 1,251,345 - BPHYT_RS25290 0.85 -2.2 1,251,437 + +1.1 1,251,521 + BPHYT_RS25295 0.26 -0.6 1,252,092 - -0.0
Or see this region's nucleotide sequence