Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25230

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25225 and BPHYT_RS25230 are separated by 96 nucleotidesBPHYT_RS25230 and BPHYT_RS25235 are separated by 138 nucleotides BPHYT_RS25225: BPHYT_RS25225 - glycine/betaine ABC transporter substrate-binding protein, at 1,233,523 to 1,234,380 _RS25225 BPHYT_RS25230: BPHYT_RS25230 - formyltetrahydrofolate deformylase, at 1,234,477 to 1,235,352 _RS25230 BPHYT_RS25235: BPHYT_RS25235 - LysR family transcriptional regulator, at 1,235,491 to 1,236,405 _RS25235 Position (kb) 1234 1235 1236Strain fitness (log2 ratio) -1 0 1at 1234.136 kb on - strand, within BPHYT_RS25225at 1234.479 kb on - strandat 1234.601 kb on + strand, within BPHYT_RS25230at 1234.817 kb on + strand, within BPHYT_RS25230at 1234.817 kb on + strand, within BPHYT_RS25230at 1235.247 kb on - strand, within BPHYT_RS25230at 1235.444 kb on - strandat 1235.710 kb on - strand, within BPHYT_RS25235at 1236.080 kb on + strand, within BPHYT_RS25235

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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1,234,136 - BPHYT_RS25225 0.71 +0.5
1,234,479 - +0.7
1,234,601 + BPHYT_RS25230 0.14 -1.5
1,234,817 + BPHYT_RS25230 0.39 -0.1
1,234,817 + BPHYT_RS25230 0.39 -1.4
1,235,247 - BPHYT_RS25230 0.88 -1.5
1,235,444 - -1.5
1,235,710 - BPHYT_RS25235 0.24 -0.2
1,236,080 + BPHYT_RS25235 0.64 -0.3

Or see this region's nucleotide sequence