Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25210

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25205 and BPHYT_RS25210 overlap by 4 nucleotidesBPHYT_RS25210 and BPHYT_RS25215 are separated by 73 nucleotides BPHYT_RS25205: BPHYT_RS25205 - electron transfer flavoprotein subunit alpha, at 1,228,764 to 1,229,945 _RS25205 BPHYT_RS25210: BPHYT_RS25210 - drug:proton antiporter, at 1,229,942 to 1,230,718 _RS25210 BPHYT_RS25215: BPHYT_RS25215 - (Fe-S)-binding protein, at 1,230,792 to 1,232,105 _RS25215 Position (kb) 1229 1230 1231Strain fitness (log2 ratio) -2 -1 0 1at 1229.198 kb on + strand, within BPHYT_RS25205at 1229.198 kb on + strand, within BPHYT_RS25205at 1229.199 kb on - strand, within BPHYT_RS25205at 1229.201 kb on - strand, within BPHYT_RS25205at 1229.316 kb on + strand, within BPHYT_RS25205at 1229.316 kb on + strand, within BPHYT_RS25205at 1229.700 kb on + strand, within BPHYT_RS25205at 1229.700 kb on + strand, within BPHYT_RS25205at 1230.455 kb on + strand, within BPHYT_RS25210

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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1,229,198 + BPHYT_RS25205 0.37 -0.7
1,229,198 + BPHYT_RS25205 0.37 -0.1
1,229,199 - BPHYT_RS25205 0.37 +0.3
1,229,201 - BPHYT_RS25205 0.37 -2.0
1,229,316 + BPHYT_RS25205 0.47 +0.1
1,229,316 + BPHYT_RS25205 0.47 -0.9
1,229,700 + BPHYT_RS25205 0.79 +1.0
1,229,700 + BPHYT_RS25205 0.79 +0.4
1,230,455 + BPHYT_RS25210 0.66 +0.8

Or see this region's nucleotide sequence