Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22540

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22535 and BPHYT_RS22540 overlap by 8 nucleotidesBPHYT_RS22540 and BPHYT_RS22545 are separated by 155 nucleotides BPHYT_RS22535: BPHYT_RS22535 - methionine ABC transporter ATP-binding protein, at 617,368 to 618,528 _RS22535 BPHYT_RS22540: BPHYT_RS22540 - metal ABC transporter permease, at 618,521 to 619,177 _RS22540 BPHYT_RS22545: BPHYT_RS22545 - acyl-CoA dehydrogenase, at 619,333 to 620,535 _RS22545 Position (kb) 618 619 620Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 617.961 kb on + strand, within BPHYT_RS22535at 618.787 kb on - strand, within BPHYT_RS22540at 618.976 kb on + strand, within BPHYT_RS22540at 619.181 kb on + strandat 619.181 kb on + strandat 619.250 kb on - strandat 619.250 kb on - strandat 619.257 kb on + strandat 619.257 kb on + strandat 619.257 kb on + strandat 619.261 kb on + strandat 619.561 kb on + strand, within BPHYT_RS22545at 619.562 kb on - strand, within BPHYT_RS22545at 619.562 kb on - strand, within BPHYT_RS22545at 620.069 kb on + strand, within BPHYT_RS22545at 620.138 kb on - strand, within BPHYT_RS22545

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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617,961 + BPHYT_RS22535 0.51 +0.5
618,787 - BPHYT_RS22540 0.40 -0.1
618,976 + BPHYT_RS22540 0.69 +0.5
619,181 + +0.9
619,181 + +0.1
619,250 - -1.6
619,250 - +0.3
619,257 + -1.4
619,257 + -0.2
619,257 + -0.5
619,261 + +2.5
619,561 + BPHYT_RS22545 0.19 -0.0
619,562 - BPHYT_RS22545 0.19 +0.5
619,562 - BPHYT_RS22545 0.19 -2.9
620,069 + BPHYT_RS22545 0.61 -0.5
620,138 - BPHYT_RS22545 0.67 -0.8

Or see this region's nucleotide sequence