Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22515 and BPHYT_RS22520 are separated by 13 nucleotides BPHYT_RS22520 and BPHYT_RS22525 are separated by 375 nucleotides
BPHYT_RS22515: BPHYT_RS22515 - multidrug efflux RND transporter permease subunit, at 609,884 to 613,069
_RS22515
BPHYT_RS22520: BPHYT_RS22520 - RND transporter, at 613,083 to 614,684
_RS22520
BPHYT_RS22525: BPHYT_RS22525 - dioxygenase, at 615,060 to 615,899
_RS22525
Position (kb) 613
614
615 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 612.209 kb on + strand, within BPHYT_RS22515 at 612.360 kb on - strand, within BPHYT_RS22515 at 612.368 kb on + strand, within BPHYT_RS22515 at 612.371 kb on - strand, within BPHYT_RS22515 at 612.498 kb on - strand, within BPHYT_RS22515 at 612.582 kb on - strand, within BPHYT_RS22515 at 612.980 kb on + strand at 612.981 kb on - strand at 613.043 kb on + strand at 613.720 kb on - strand, within BPHYT_RS22520 at 613.759 kb on - strand, within BPHYT_RS22520 at 613.759 kb on - strand, within BPHYT_RS22520 at 614.445 kb on + strand, within BPHYT_RS22520 at 614.467 kb on - strand, within BPHYT_RS22520 at 614.467 kb on - strand, within BPHYT_RS22520 at 614.790 kb on + strand at 614.814 kb on - strand at 615.293 kb on + strand, within BPHYT_RS22525 at 615.337 kb on - strand, within BPHYT_RS22525 at 615.348 kb on - strand, within BPHYT_RS22525 at 615.384 kb on + strand, within BPHYT_RS22525 at 615.432 kb on + strand, within BPHYT_RS22525
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 612,209 + BPHYT_RS22515 0.73 +0.2 612,360 - BPHYT_RS22515 0.78 +0.5 612,368 + BPHYT_RS22515 0.78 +1.2 612,371 - BPHYT_RS22515 0.78 -0.7 612,498 - BPHYT_RS22515 0.82 +1.0 612,582 - BPHYT_RS22515 0.85 -0.7 612,980 + -2.1 612,981 - -0.7 613,043 + +0.7 613,720 - BPHYT_RS22520 0.40 -2.2 613,759 - BPHYT_RS22520 0.42 +2.9 613,759 - BPHYT_RS22520 0.42 -1.6 614,445 + BPHYT_RS22520 0.85 -0.4 614,467 - BPHYT_RS22520 0.86 -1.4 614,467 - BPHYT_RS22520 0.86 +0.2 614,790 + +0.7 614,814 - -1.4 615,293 + BPHYT_RS22525 0.28 +0.6 615,337 - BPHYT_RS22525 0.33 -0.8 615,348 - BPHYT_RS22525 0.34 -1.6 615,384 + BPHYT_RS22525 0.39 +0.5 615,432 + BPHYT_RS22525 0.44 -1.6
Or see this region's nucleotide sequence