Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21545 and BPHYT_RS21550 are separated by 168 nucleotides BPHYT_RS21550 and BPHYT_RS21555 are separated by 210 nucleotides BPHYT_RS21555 and BPHYT_RS21560 are separated by 258 nucleotides
BPHYT_RS21545: BPHYT_RS21545 - SAM-dependent methyltransferase, at 392,706 to 393,422
_RS21545
BPHYT_RS21550: BPHYT_RS21550 - hypothetical protein, at 393,591 to 394,109
_RS21550
BPHYT_RS21555: BPHYT_RS21555 - beta alanine--pyruvate aminotransferase, at 394,320 to 395,648
_RS21555
BPHYT_RS21560: BPHYT_RS21560 - GntR family transcriptional regulator, at 395,907 to 397,322
_RS21560
Position (kb) 394
395
396 Strain fitness (log2 ratio) -2
-1
0
1
2 at 393.333 kb on + strand, within BPHYT_RS21545 at 394.113 kb on - strand at 394.220 kb on + strand at 394.241 kb on + strand at 394.321 kb on + strand at 394.322 kb on - strand at 394.633 kb on + strand, within BPHYT_RS21555 at 394.684 kb on + strand, within BPHYT_RS21555 at 394.837 kb on + strand, within BPHYT_RS21555 at 395.536 kb on + strand at 395.539 kb on + strand at 395.540 kb on - strand at 395.651 kb on + strand at 395.652 kb on - strand at 395.724 kb on + strand at 395.724 kb on + strand at 395.724 kb on + strand at 395.835 kb on + strand at 395.835 kb on + strand at 395.860 kb on - strand at 395.860 kb on - strand at 395.860 kb on - strand at 395.917 kb on + strand at 395.918 kb on - strand at 396.084 kb on + strand, within BPHYT_RS21560 at 396.085 kb on - strand, within BPHYT_RS21560 at 396.085 kb on - strand, within BPHYT_RS21560 at 396.354 kb on + strand, within BPHYT_RS21560
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 393,333 + BPHYT_RS21545 0.87 -0.4 394,113 - -0.9 394,220 + +0.2 394,241 + -0.9 394,321 + -0.4 394,322 - +0.8 394,633 + BPHYT_RS21555 0.24 +1.0 394,684 + BPHYT_RS21555 0.27 -0.1 394,837 + BPHYT_RS21555 0.39 +1.0 395,536 + +0.1 395,539 + -0.4 395,540 - +0.0 395,651 + +0.9 395,652 - +0.8 395,724 + -0.9 395,724 + +0.0 395,724 + -0.6 395,835 + -0.2 395,835 + +0.3 395,860 - -1.3 395,860 - +2.3 395,860 - +0.1 395,917 + -1.9 395,918 - -0.1 396,084 + BPHYT_RS21560 0.12 -0.6 396,085 - BPHYT_RS21560 0.13 -0.1 396,085 - BPHYT_RS21560 0.13 -0.1 396,354 + BPHYT_RS21560 0.32 -0.1
Or see this region's nucleotide sequence