Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21105 and BPHYT_RS21110 are separated by 67 nucleotides BPHYT_RS21110 and BPHYT_RS21115 are separated by 32 nucleotides
BPHYT_RS21105: BPHYT_RS21105 - phenylhydantoinase, at 296,186 to 297,607
_RS21105
BPHYT_RS21110: BPHYT_RS21110 - FO synthase, at 297,675 to 298,835
_RS21110
BPHYT_RS21115: BPHYT_RS21115 - radical SAM protein, at 298,868 to 300,139
_RS21115
Position (kb) 297
298
299 Strain fitness (log2 ratio) -2
-1
0
1
2 at 297.119 kb on - strand, within BPHYT_RS21105 at 297.119 kb on - strand, within BPHYT_RS21105 at 297.316 kb on + strand, within BPHYT_RS21105 at 297.317 kb on - strand, within BPHYT_RS21105 at 297.631 kb on - strand at 297.675 kb on - strand at 297.789 kb on + strand at 297.790 kb on - strand at 297.982 kb on + strand, within BPHYT_RS21110 at 298.039 kb on - strand, within BPHYT_RS21110 at 298.502 kb on + strand, within BPHYT_RS21110 at 298.705 kb on - strand, within BPHYT_RS21110 at 299.046 kb on + strand, within BPHYT_RS21115 at 299.117 kb on + strand, within BPHYT_RS21115 at 299.356 kb on - strand, within BPHYT_RS21115 at 299.361 kb on - strand, within BPHYT_RS21115 at 299.410 kb on + strand, within BPHYT_RS21115 at 299.555 kb on - strand, within BPHYT_RS21115 at 299.571 kb on - strand, within BPHYT_RS21115 at 299.696 kb on + strand, within BPHYT_RS21115 at 299.697 kb on - strand, within BPHYT_RS21115 at 299.749 kb on + strand, within BPHYT_RS21115 at 299.750 kb on - strand, within BPHYT_RS21115 at 299.823 kb on + strand, within BPHYT_RS21115
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 297,119 - BPHYT_RS21105 0.66 +0.3 297,119 - BPHYT_RS21105 0.66 -0.3 297,316 + BPHYT_RS21105 0.79 -0.5 297,317 - BPHYT_RS21105 0.80 -0.4 297,631 - +1.2 297,675 - -1.6 297,789 + +2.0 297,790 - -1.2 297,982 + BPHYT_RS21110 0.26 -1.5 298,039 - BPHYT_RS21110 0.31 -0.3 298,502 + BPHYT_RS21110 0.71 +2.0 298,705 - BPHYT_RS21110 0.89 -1.9 299,046 + BPHYT_RS21115 0.14 +0.5 299,117 + BPHYT_RS21115 0.20 -1.5 299,356 - BPHYT_RS21115 0.38 +0.3 299,361 - BPHYT_RS21115 0.39 +0.7 299,410 + BPHYT_RS21115 0.43 +1.2 299,555 - BPHYT_RS21115 0.54 +0.3 299,571 - BPHYT_RS21115 0.55 -0.3 299,696 + BPHYT_RS21115 0.65 +0.5 299,697 - BPHYT_RS21115 0.65 +0.2 299,749 + BPHYT_RS21115 0.69 -0.0 299,750 - BPHYT_RS21115 0.69 +0.4 299,823 + BPHYT_RS21115 0.75 +0.3
Or see this region's nucleotide sequence