Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16015 and BPHYT_RS16020 are separated by 18 nucleotides BPHYT_RS16020 and BPHYT_RS16025 are separated by 130 nucleotides BPHYT_RS16025 and BPHYT_RS16030 are separated by 28 nucleotides
BPHYT_RS16015: BPHYT_RS16015 - flavin reductase, at 3,634,169 to 3,634,678
_RS16015
BPHYT_RS16020: BPHYT_RS16020 - AsnC family transcriptional regulator, at 3,634,697 to 3,635,212
_RS16020
BPHYT_RS16025: BPHYT_RS16025 - Kynurenine formamidase, bacterial (EC 3.5.1.9) (from data) , at 3,635,343 to 3,635,981
_RS16025
BPHYT_RS16030: BPHYT_RS16030 - Kynureninase (EC 3.7.1.3) (from data) , at 3,636,010 to 3,637,260
_RS16030
Position (kb)
3635
3636 Strain fitness (log2 ratio)
-2
-1
0
1 at 3634.391 kb on + strand, within BPHYT_RS16015 at 3634.392 kb on - strand, within BPHYT_RS16015 at 3634.541 kb on - strand, within BPHYT_RS16015 at 3634.888 kb on - strand, within BPHYT_RS16020 at 3635.035 kb on + strand, within BPHYT_RS16020 at 3635.049 kb on + strand, within BPHYT_RS16020 at 3635.343 kb on - strand at 3635.517 kb on + strand, within BPHYT_RS16025 at 3635.517 kb on + strand, within BPHYT_RS16025 at 3635.518 kb on - strand, within BPHYT_RS16025 at 3635.663 kb on - strand, within BPHYT_RS16025 at 3635.673 kb on + strand, within BPHYT_RS16025 at 3636.112 kb on - strand at 3636.410 kb on - strand, within BPHYT_RS16030 at 3636.449 kb on - strand, within BPHYT_RS16030 at 3636.686 kb on - strand, within BPHYT_RS16030 at 3636.687 kb on + strand, within BPHYT_RS16030 at 3636.687 kb on + strand, within BPHYT_RS16030 at 3636.688 kb on - strand, within BPHYT_RS16030 at 3636.688 kb on - strand, within BPHYT_RS16030 at 3636.692 kb on - strand, within BPHYT_RS16030 at 3636.735 kb on + strand, within BPHYT_RS16030 at 3636.735 kb on + strand, within BPHYT_RS16030
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 3,634,391 + BPHYT_RS16015 0.44 -0.8 3,634,392 - BPHYT_RS16015 0.44 +0.4 3,634,541 - BPHYT_RS16015 0.73 -1.5 3,634,888 - BPHYT_RS16020 0.37 -0.2 3,635,035 + BPHYT_RS16020 0.66 +1.0 3,635,049 + BPHYT_RS16020 0.68 +1.2 3,635,343 - -0.4 3,635,517 + BPHYT_RS16025 0.27 -2.0 3,635,517 + BPHYT_RS16025 0.27 -1.8 3,635,518 - BPHYT_RS16025 0.27 -1.2 3,635,663 - BPHYT_RS16025 0.50 -0.6 3,635,673 + BPHYT_RS16025 0.52 -0.3 3,636,112 - +1.3 3,636,410 - BPHYT_RS16030 0.32 +1.4 3,636,449 - BPHYT_RS16030 0.35 +0.7 3,636,686 - BPHYT_RS16030 0.54 +0.3 3,636,687 + BPHYT_RS16030 0.54 -1.3 3,636,687 + BPHYT_RS16030 0.54 -1.1 3,636,688 - BPHYT_RS16030 0.54 -0.0 3,636,688 - BPHYT_RS16030 0.54 -2.4 3,636,692 - BPHYT_RS16030 0.55 +0.7 3,636,735 + BPHYT_RS16030 0.58 -1.0 3,636,735 + BPHYT_RS16030 0.58 -0.3
Or see this region's nucleotide sequence