Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotides BPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotides BPHYT_RS14415 and BPHYT_RS14420 are separated by 232 nucleotides BPHYT_RS14420 and BPHYT_RS14425 are separated by 152 nucleotides
BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147
_RS14405
BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632
_RS14410
BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988
_RS14415
BPHYT_RS14420: BPHYT_RS14420 - acyl carrier protein, at 3,290,221 to 3,290,460
_RS14420
BPHYT_RS14425: BPHYT_RS14425 - 3-ketoacyl-ACP reductase, at 3,290,613 to 3,291,362
_RS14425
Position (kb)
3288
3289
3290 Strain fitness (log2 ratio)
-1
0
1 at 3288.193 kb on - strand at 3288.193 kb on - strand at 3288.218 kb on - strand at 3288.218 kb on - strand at 3288.324 kb on + strand, within BPHYT_RS14410 at 3288.494 kb on - strand, within BPHYT_RS14410 at 3288.546 kb on - strand, within BPHYT_RS14410 at 3288.583 kb on + strand, within BPHYT_RS14410 at 3290.568 kb on - strand at 3290.576 kb on - strand at 3290.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 3,288,193 - -1.3 3,288,193 - +0.9 3,288,218 - +0.7 3,288,218 - +0.5 3,288,324 + BPHYT_RS14410 0.20 +0.9 3,288,494 - BPHYT_RS14410 0.64 +1.0 3,288,546 - BPHYT_RS14410 0.77 +0.4 3,288,583 + BPHYT_RS14410 0.87 -0.7 3,290,568 - +0.5 3,290,576 - -0.1 3,290,598 - -1.1
Or see this region's nucleotide sequence