Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14405

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14395 and BPHYT_RS14400 are separated by 4 nucleotidesBPHYT_RS14400 and BPHYT_RS14405 are separated by 83 nucleotidesBPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotidesBPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotides BPHYT_RS14395: BPHYT_RS14395 - sugar dehydratase, at 3,285,787 to 3,286,830 _RS14395 BPHYT_RS14400: BPHYT_RS14400 - anti-sigma factor, at 3,286,835 to 3,287,464 _RS14400 BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147 _RS14405 BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632 _RS14410 BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988 _RS14415 Position (kb) 3287 3288 3289Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3286.746 kb on + strandat 3286.801 kb on - strandat 3286.836 kb on + strandat 3286.836 kb on + strandat 3286.930 kb on - strand, within BPHYT_RS14400at 3287.314 kb on - strand, within BPHYT_RS14400at 3287.314 kb on - strand, within BPHYT_RS14400at 3287.357 kb on - strand, within BPHYT_RS14400at 3287.479 kb on + strandat 3287.479 kb on + strandat 3287.480 kb on - strandat 3287.480 kb on - strandat 3287.485 kb on + strandat 3287.485 kb on + strandat 3287.486 kb on - strandat 3287.514 kb on - strandat 3288.193 kb on - strandat 3288.193 kb on - strandat 3288.218 kb on - strandat 3288.218 kb on - strandat 3288.324 kb on + strand, within BPHYT_RS14410at 3288.494 kb on - strand, within BPHYT_RS14410at 3288.546 kb on - strand, within BPHYT_RS14410at 3288.583 kb on + strand, within BPHYT_RS14410

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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3,286,746 + +1.5
3,286,801 - +1.3
3,286,836 + +1.8
3,286,836 + +1.0
3,286,930 - BPHYT_RS14400 0.15 +1.4
3,287,314 - BPHYT_RS14400 0.76 +1.8
3,287,314 - BPHYT_RS14400 0.76 +2.2
3,287,357 - BPHYT_RS14400 0.83 +2.5
3,287,479 + +2.9
3,287,479 + +1.8
3,287,480 - -2.1
3,287,480 - +1.1
3,287,485 + +2.9
3,287,485 + +1.7
3,287,486 - +0.4
3,287,514 - +1.9
3,288,193 - -1.3
3,288,193 - +0.9
3,288,218 - +0.7
3,288,218 - +0.5
3,288,324 + BPHYT_RS14410 0.20 +0.9
3,288,494 - BPHYT_RS14410 0.64 +1.0
3,288,546 - BPHYT_RS14410 0.77 +0.4
3,288,583 + BPHYT_RS14410 0.87 -0.7

Or see this region's nucleotide sequence