Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13810

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13805 and BPHYT_RS13810 are separated by 31 nucleotides BPHYT_RS13805: BPHYT_RS13805 - type VI secretion protein ImpA, at 3,145,358 to 3,147,754 _RS13805 BPHYT_RS13810: BPHYT_RS13810 - type VI secretion protein, at 3,147,786 to 3,149,714 _RS13810 Position (kb) 3147 3148 3149 3150Strain fitness (log2 ratio) -2 -1 0 1at 3146.860 kb on - strand, within BPHYT_RS13805at 3146.947 kb on + strand, within BPHYT_RS13805at 3147.014 kb on - strand, within BPHYT_RS13805at 3147.159 kb on - strand, within BPHYT_RS13805at 3147.515 kb on - strandat 3147.925 kb on + strandat 3148.508 kb on - strand, within BPHYT_RS13810at 3148.891 kb on + strand, within BPHYT_RS13810at 3149.226 kb on + strand, within BPHYT_RS13810at 3149.226 kb on + strand, within BPHYT_RS13810at 3149.226 kb on + strand, within BPHYT_RS13810at 3149.342 kb on + strandat 3149.444 kb on - strand, within BPHYT_RS13810at 3149.665 kb on + strandat 3150.106 kb on + strandat 3150.111 kb on - strandat 3150.137 kb on + strandat 3150.225 kb on + strandat 3150.225 kb on + strandat 3150.225 kb on + strandat 3150.459 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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3,146,860 - BPHYT_RS13805 0.63 -0.7
3,146,947 + BPHYT_RS13805 0.66 -1.0
3,147,014 - BPHYT_RS13805 0.69 -0.0
3,147,159 - BPHYT_RS13805 0.75 -0.6
3,147,515 - -2.1
3,147,925 + -1.1
3,148,508 - BPHYT_RS13810 0.37 +0.1
3,148,891 + BPHYT_RS13810 0.57 -0.2
3,149,226 + BPHYT_RS13810 0.75 -1.2
3,149,226 + BPHYT_RS13810 0.75 -0.9
3,149,226 + BPHYT_RS13810 0.75 -0.3
3,149,342 + +0.3
3,149,444 - BPHYT_RS13810 0.86 -2.6
3,149,665 + +0.3
3,150,106 + -0.9
3,150,111 - +0.2
3,150,137 + -0.7
3,150,225 + -0.9
3,150,225 + -1.3
3,150,225 + +1.0
3,150,459 + -1.7

Or see this region's nucleotide sequence