Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13180 and BPHYT_RS13185 are separated by 339 nucleotides BPHYT_RS13185 and BPHYT_RS13190 are separated by 55 nucleotides
BPHYT_RS13180: BPHYT_RS13180 - hypothetical protein, at 3,007,335 to 3,008,129
_RS13180
BPHYT_RS13185: BPHYT_RS13185 - 2,4-dienoyl-CoA reductase, at 3,008,469 to 3,010,517
_RS13185
BPHYT_RS13190: BPHYT_RS13190 - membrane protein, at 3,010,573 to 3,011,502
_RS13190
Position (kb)
3008
3009
3010
3011 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3007.550 kb on - strand, within BPHYT_RS13180 at 3007.567 kb on + strand, within BPHYT_RS13180 at 3007.567 kb on + strand, within BPHYT_RS13180 at 3007.567 kb on + strand, within BPHYT_RS13180 at 3007.724 kb on + strand, within BPHYT_RS13180 at 3007.904 kb on + strand, within BPHYT_RS13180 at 3007.928 kb on + strand, within BPHYT_RS13180 at 3007.930 kb on + strand, within BPHYT_RS13180 at 3007.931 kb on - strand, within BPHYT_RS13180 at 3007.931 kb on - strand, within BPHYT_RS13180 at 3008.143 kb on - strand at 3008.143 kb on - strand at 3008.225 kb on - strand at 3008.450 kb on + strand at 3008.455 kb on - strand at 3008.455 kb on - strand at 3008.476 kb on - strand at 3009.109 kb on - strand, within BPHYT_RS13185 at 3009.217 kb on + strand, within BPHYT_RS13185 at 3009.933 kb on + strand, within BPHYT_RS13185 at 3009.933 kb on + strand, within BPHYT_RS13185 at 3010.718 kb on + strand, within BPHYT_RS13190 at 3010.718 kb on + strand, within BPHYT_RS13190 at 3010.808 kb on + strand, within BPHYT_RS13190 at 3011.308 kb on + strand, within BPHYT_RS13190 at 3011.384 kb on + strand, within BPHYT_RS13190
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 3,007,550 - BPHYT_RS13180 0.27 -0.1 3,007,567 + BPHYT_RS13180 0.29 +0.8 3,007,567 + BPHYT_RS13180 0.29 -1.3 3,007,567 + BPHYT_RS13180 0.29 +0.4 3,007,724 + BPHYT_RS13180 0.49 -0.7 3,007,904 + BPHYT_RS13180 0.72 -0.4 3,007,928 + BPHYT_RS13180 0.75 -0.5 3,007,930 + BPHYT_RS13180 0.75 +1.3 3,007,931 - BPHYT_RS13180 0.75 -2.5 3,007,931 - BPHYT_RS13180 0.75 +0.2 3,008,143 - +0.7 3,008,143 - -0.4 3,008,225 - +0.5 3,008,450 + +1.0 3,008,455 - -2.8 3,008,455 - -0.4 3,008,476 - -0.9 3,009,109 - BPHYT_RS13185 0.31 -0.8 3,009,217 + BPHYT_RS13185 0.37 +0.1 3,009,933 + BPHYT_RS13185 0.71 -2.0 3,009,933 + BPHYT_RS13185 0.71 -3.4 3,010,718 + BPHYT_RS13190 0.16 -0.9 3,010,718 + BPHYT_RS13190 0.16 +0.8 3,010,808 + BPHYT_RS13190 0.25 -2.0 3,011,308 + BPHYT_RS13190 0.79 +0.2 3,011,384 + BPHYT_RS13190 0.87 -0.1
Or see this region's nucleotide sequence