Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08150

Experiment: 24hr PHYTO7 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08145 and BPHYT_RS08150 are separated by 178 nucleotidesBPHYT_RS08150 and BPHYT_RS08155 are separated by 655 nucleotidesBPHYT_RS08155 and BPHYT_RS08160 are separated by 140 nucleotides BPHYT_RS08145: BPHYT_RS08145 - membrane protein, at 1,862,590 to 1,864,005 _RS08145 BPHYT_RS08150: BPHYT_RS08150 - membrane protein, at 1,864,184 to 1,865,683 _RS08150 BPHYT_RS08155: BPHYT_RS08155 - pilus biosynthesis protein, at 1,866,339 to 1,866,518 _RS08155 BPHYT_RS08160: BPHYT_RS08160 - membrane protein, at 1,866,659 to 1,867,159 _RS08160 Position (kb) 1864 1865 1866Strain fitness (log2 ratio) -2 -1 0 1at 1864.159 kb on - strandat 1864.185 kb on + strandat 1864.655 kb on - strand, within BPHYT_RS08150at 1865.925 kb on - strandat 1866.052 kb on - strandat 1866.128 kb on - strandat 1866.390 kb on - strand, within BPHYT_RS08155at 1866.517 kb on - strandat 1866.530 kb on + strandat 1866.583 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1
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1,864,159 - -0.3
1,864,185 + +0.6
1,864,655 - BPHYT_RS08150 0.31 -1.9
1,865,925 - -0.3
1,866,052 - +0.4
1,866,128 - -0.1
1,866,390 - BPHYT_RS08155 0.28 -0.1
1,866,517 - -0.1
1,866,530 + -0.1
1,866,583 + -0.3

Or see this region's nucleotide sequence