Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07240 and BPHYT_RS07245 are separated by 11 nucleotides BPHYT_RS07245 and BPHYT_RS07250 are separated by 13 nucleotides BPHYT_RS07250 and BPHYT_RS07255 overlap by 4 nucleotides BPHYT_RS07255 and BPHYT_RS07260 overlap by 4 nucleotides
BPHYT_RS07240: BPHYT_RS07240 - 2-keto-4-pentenoate hydratase, at 1,644,639 to 1,645,460
_RS07240
BPHYT_RS07245: BPHYT_RS07245 - acetaldehyde dehydrogenase, at 1,645,472 to 1,646,416
_RS07245
BPHYT_RS07250: BPHYT_RS07250 - 4-hydroxy-2-oxovalerate aldolase, at 1,646,430 to 1,647,473
_RS07250
BPHYT_RS07255: BPHYT_RS07255 - 4-oxalocrotonate decarboxylase, at 1,647,470 to 1,648,243
_RS07255
BPHYT_RS07260: BPHYT_RS07260 - 2-aminomuconate deaminase, at 1,648,240 to 1,648,671
_RS07260
Position (kb) 1646
1647
1648 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1646.336 kb on + strand at 1646.565 kb on - strand, within BPHYT_RS07250 at 1646.858 kb on + strand, within BPHYT_RS07250 at 1646.926 kb on - strand, within BPHYT_RS07250 at 1647.339 kb on + strand, within BPHYT_RS07250 at 1647.650 kb on - strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.670 kb on - strand, within BPHYT_RS07255 at 1648.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 1,646,336 + -0.6 1,646,565 - BPHYT_RS07250 0.13 -1.2 1,646,858 + BPHYT_RS07250 0.41 -0.6 1,646,926 - BPHYT_RS07250 0.48 -0.4 1,647,339 + BPHYT_RS07250 0.87 -1.3 1,647,650 - BPHYT_RS07255 0.23 -1.0 1,647,669 + BPHYT_RS07255 0.26 -1.9 1,647,669 + BPHYT_RS07255 0.26 +0.5 1,647,670 - BPHYT_RS07255 0.26 +2.3 1,648,230 - -2.0
Or see this region's nucleotide sequence