Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07080 and BPHYT_RS07085 are separated by 231 nucleotides BPHYT_RS07085 and BPHYT_RS07090 are separated by 127 nucleotides BPHYT_RS07090 and BPHYT_RS07095 overlap by 31 nucleotides
BPHYT_RS07080: BPHYT_RS07080 - glutathione S-transferase, at 1,600,331 to 1,601,341
_RS07080
BPHYT_RS07085: BPHYT_RS07085 - LysR family transcriptional regulator, at 1,601,573 to 1,602,496
_RS07085
BPHYT_RS07090: BPHYT_RS07090 - hypothetical protein, at 1,602,624 to 1,602,941
_RS07090
BPHYT_RS07095: BPHYT_RS07095 - LysR family transcriptional regulator, at 1,602,911 to 1,603,837
_RS07095
Position (kb) 1601
1602
1603 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1600.728 kb on + strand, within BPHYT_RS07080 at 1601.170 kb on - strand, within BPHYT_RS07080 at 1601.301 kb on + strand at 1601.331 kb on - strand at 1601.439 kb on + strand at 1601.508 kb on + strand at 1601.509 kb on - strand at 1602.200 kb on + strand, within BPHYT_RS07085 at 1602.377 kb on + strand at 1602.565 kb on + strand at 1602.565 kb on + strand at 1602.901 kb on + strand, within BPHYT_RS07090 at 1602.913 kb on - strand at 1602.978 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 1,600,728 + BPHYT_RS07080 0.39 -0.2 1,601,170 - BPHYT_RS07080 0.83 +0.6 1,601,301 + +0.9 1,601,331 - +0.7 1,601,439 + +1.0 1,601,508 + -0.4 1,601,509 - +0.3 1,602,200 + BPHYT_RS07085 0.68 +0.4 1,602,377 + +2.3 1,602,565 + +0.1 1,602,565 + +1.0 1,602,901 + BPHYT_RS07090 0.87 -0.8 1,602,913 - +0.3 1,602,978 + -1.9
Or see this region's nucleotide sequence