Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04325 and BPHYT_RS04330 are separated by 125 nucleotides BPHYT_RS04330 and BPHYT_RS04335 are separated by 53 nucleotides BPHYT_RS04335 and BPHYT_RS04340 overlap by 4 nucleotides
BPHYT_RS04325: BPHYT_RS04325 - acyltransferase 3, at 995,925 to 997,088
_RS04325
BPHYT_RS04330: BPHYT_RS04330 - hypothetical protein, at 997,214 to 998,356
_RS04330
BPHYT_RS04335: BPHYT_RS04335 - membrane protein, at 998,410 to 998,835
_RS04335
BPHYT_RS04340: BPHYT_RS04340 - hypothetical protein, at 998,832 to 1,000,115
_RS04340
Position (kb) 997
998
999 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 996.301 kb on - strand, within BPHYT_RS04325 at 996.363 kb on + strand, within BPHYT_RS04325 at 996.487 kb on + strand, within BPHYT_RS04325 at 996.509 kb on + strand, within BPHYT_RS04325 at 996.540 kb on - strand, within BPHYT_RS04325 at 996.705 kb on + strand, within BPHYT_RS04325 at 996.716 kb on + strand, within BPHYT_RS04325 at 996.873 kb on + strand, within BPHYT_RS04325 at 997.086 kb on + strand at 997.113 kb on + strand at 997.114 kb on - strand at 997.203 kb on + strand at 997.204 kb on - strand at 997.204 kb on - strand at 997.207 kb on + strand at 997.239 kb on - strand at 997.352 kb on + strand, within BPHYT_RS04330 at 997.352 kb on + strand, within BPHYT_RS04330 at 997.353 kb on - strand, within BPHYT_RS04330 at 997.353 kb on - strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.446 kb on - strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 998.117 kb on + strand, within BPHYT_RS04330 at 998.203 kb on + strand, within BPHYT_RS04330 at 998.203 kb on + strand, within BPHYT_RS04330 at 998.204 kb on - strand, within BPHYT_RS04330 at 998.215 kb on - strand, within BPHYT_RS04330 at 998.215 kb on - strand, within BPHYT_RS04330 at 998.528 kb on + strand, within BPHYT_RS04335 at 998.528 kb on + strand, within BPHYT_RS04335 at 998.529 kb on - strand, within BPHYT_RS04335 at 998.836 kb on - strand at 998.836 kb on - strand at 998.899 kb on + strand at 998.900 kb on - strand at 998.900 kb on - strand at 998.902 kb on + strand at 998.965 kb on + strand, within BPHYT_RS04340 at 999.212 kb on + strand, within BPHYT_RS04340 at 999.343 kb on + strand, within BPHYT_RS04340
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 996,301 - BPHYT_RS04325 0.32 -2.6 996,363 + BPHYT_RS04325 0.38 -0.9 996,487 + BPHYT_RS04325 0.48 +1.7 996,509 + BPHYT_RS04325 0.50 -2.2 996,540 - BPHYT_RS04325 0.53 -0.9 996,705 + BPHYT_RS04325 0.67 +0.2 996,716 + BPHYT_RS04325 0.68 -0.8 996,873 + BPHYT_RS04325 0.81 -2.0 997,086 + +0.4 997,113 + -2.0 997,114 - +0.3 997,203 + -2.3 997,204 - +0.0 997,204 - -0.8 997,207 + -0.8 997,239 - -1.6 997,352 + BPHYT_RS04330 0.12 -0.5 997,352 + BPHYT_RS04330 0.12 -1.1 997,353 - BPHYT_RS04330 0.12 -1.3 997,353 - BPHYT_RS04330 0.12 -4.8 997,360 + BPHYT_RS04330 0.13 -0.7 997,360 + BPHYT_RS04330 0.13 -0.8 997,360 + BPHYT_RS04330 0.13 -1.1 997,446 - BPHYT_RS04330 0.20 -2.3 997,576 + BPHYT_RS04330 0.32 -0.5 997,576 + BPHYT_RS04330 0.32 -1.3 997,576 + BPHYT_RS04330 0.32 -0.7 998,117 + BPHYT_RS04330 0.79 -1.6 998,203 + BPHYT_RS04330 0.87 -3.1 998,203 + BPHYT_RS04330 0.87 -1.4 998,204 - BPHYT_RS04330 0.87 -1.8 998,215 - BPHYT_RS04330 0.88 -1.2 998,215 - BPHYT_RS04330 0.88 -1.0 998,528 + BPHYT_RS04335 0.28 -0.4 998,528 + BPHYT_RS04335 0.28 +0.3 998,529 - BPHYT_RS04335 0.28 -0.4 998,836 - -2.1 998,836 - -1.7 998,899 + +1.3 998,900 - -0.4 998,900 - +1.9 998,902 + +0.6 998,965 + BPHYT_RS04340 0.10 +0.9 999,212 + BPHYT_RS04340 0.30 +0.9 999,343 + BPHYT_RS04340 0.40 +1.9
Or see this region's nucleotide sequence