Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02565 and BPHYT_RS02570 are separated by 96 nucleotides BPHYT_RS02570 and BPHYT_RS02575 are separated by 305 nucleotides BPHYT_RS02575 and BPHYT_RS02580 are separated by 87 nucleotides
BPHYT_RS02565: BPHYT_RS02565 - PTS mannose transporter subunit IIA, at 582,866 to 583,393
_RS02565
BPHYT_RS02570: BPHYT_RS02570 - phosphocarrier protein HPr, at 583,490 to 583,759
_RS02570
BPHYT_RS02575: BPHYT_RS02575 - phosphoenolpyruvate-protein phosphotransferase, at 584,065 to 585,813
_RS02575
BPHYT_RS02580: BPHYT_RS02580 - molybdopterin biosynthesis protein MoeB, at 585,901 to 586,659
_RS02580
Position (kb)
584
585
586 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 583.389 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.391 kb on + strand at 583.392 kb on - strand at 583.392 kb on - strand at 583.449 kb on + strand at 583.468 kb on + strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.511 kb on - strand at 583.574 kb on + strand, within BPHYT_RS02570 at 583.575 kb on - strand, within BPHYT_RS02570 at 583.575 kb on - strand, within BPHYT_RS02570 at 583.994 kb on + strand at 583.995 kb on - strand at 584.051 kb on + strand at 584.052 kb on - strand at 584.052 kb on - strand at 584.150 kb on + strand at 584.158 kb on + strand at 584.158 kb on + strand at 584.159 kb on - strand at 584.227 kb on + strand at 584.437 kb on + strand, within BPHYT_RS02575 at 584.736 kb on + strand, within BPHYT_RS02575 at 584.797 kb on + strand, within BPHYT_RS02575 at 584.798 kb on - strand, within BPHYT_RS02575 at 584.798 kb on - strand, within BPHYT_RS02575 at 584.836 kb on - strand, within BPHYT_RS02575 at 584.836 kb on - strand, within BPHYT_RS02575 at 584.836 kb on - strand, within BPHYT_RS02575 at 584.994 kb on + strand, within BPHYT_RS02575 at 584.995 kb on - strand, within BPHYT_RS02575 at 584.995 kb on - strand, within BPHYT_RS02575 at 585.355 kb on + strand, within BPHYT_RS02575 at 585.355 kb on + strand, within BPHYT_RS02575 at 585.473 kb on - strand, within BPHYT_RS02575 at 585.517 kb on + strand, within BPHYT_RS02575 at 585.517 kb on + strand, within BPHYT_RS02575 at 585.871 kb on + strand at 585.871 kb on + strand at 585.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 583,389 + +0.9 583,391 + +1.4 583,391 + +1.4 583,391 + +0.3 583,392 - +0.2 583,392 - -0.4 583,449 + +0.4 583,468 + +1.1 583,511 - -1.6 583,511 - -2.6 583,511 - -0.9 583,574 + BPHYT_RS02570 0.31 -0.6 583,575 - BPHYT_RS02570 0.31 -1.4 583,575 - BPHYT_RS02570 0.31 -2.0 583,994 + +0.1 583,995 - +0.3 584,051 + +0.3 584,052 - -1.3 584,052 - -1.4 584,150 + -1.5 584,158 + -0.7 584,158 + -1.2 584,159 - -1.9 584,227 + -1.8 584,437 + BPHYT_RS02575 0.21 -1.5 584,736 + BPHYT_RS02575 0.38 +0.4 584,797 + BPHYT_RS02575 0.42 -1.1 584,798 - BPHYT_RS02575 0.42 -4.6 584,798 - BPHYT_RS02575 0.42 -1.5 584,836 - BPHYT_RS02575 0.44 -1.5 584,836 - BPHYT_RS02575 0.44 -3.0 584,836 - BPHYT_RS02575 0.44 +0.3 584,994 + BPHYT_RS02575 0.53 -0.9 584,995 - BPHYT_RS02575 0.53 -0.3 584,995 - BPHYT_RS02575 0.53 -2.0 585,355 + BPHYT_RS02575 0.74 -1.1 585,355 + BPHYT_RS02575 0.74 -1.1 585,473 - BPHYT_RS02575 0.81 -2.3 585,517 + BPHYT_RS02575 0.83 -0.5 585,517 + BPHYT_RS02575 0.83 -1.3 585,871 + +0.6 585,871 + +1.9 585,872 - -0.2
Or see this region's nucleotide sequence