Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00805 and BPHYT_RS00810 overlap by 4 nucleotides BPHYT_RS00810 and BPHYT_RS00815 are separated by 14 nucleotides BPHYT_RS00815 and BPHYT_RS00820 overlap by 4 nucleotides
BPHYT_RS00805: BPHYT_RS00805 - type IV secretion system protein VirB10, at 181,925 to 183,127
_RS00805
BPHYT_RS00810: BPHYT_RS00810 - type IV secretion system protein VirB11, at 183,124 to 184,251
_RS00810
BPHYT_RS00815: BPHYT_RS00815 - hypothetical protein, at 184,266 to 184,676
_RS00815
BPHYT_RS00820: BPHYT_RS00820 - type IV secretory pathway protein VirD4, at 184,673 to 186,457
_RS00820
Position (kb) 183
184
185 Strain fitness (log2 ratio) -2
-1
0
1
2 at 182.182 kb on - strand, within BPHYT_RS00805 at 182.242 kb on + strand at 182.292 kb on + strand, within BPHYT_RS00805 at 182.339 kb on + strand, within BPHYT_RS00805 at 182.340 kb on - strand, within BPHYT_RS00805 at 182.340 kb on - strand, within BPHYT_RS00805 at 182.343 kb on - strand, within BPHYT_RS00805 at 182.635 kb on + strand, within BPHYT_RS00805 at 182.844 kb on - strand, within BPHYT_RS00805 at 182.844 kb on - strand, within BPHYT_RS00805 at 182.958 kb on - strand, within BPHYT_RS00805 at 182.958 kb on - strand, within BPHYT_RS00805 at 183.098 kb on + strand at 183.099 kb on - strand at 183.099 kb on - strand at 183.171 kb on + strand at 183.172 kb on - strand at 183.638 kb on - strand, within BPHYT_RS00810 at 183.697 kb on - strand, within BPHYT_RS00810 at 183.714 kb on + strand, within BPHYT_RS00810 at 183.844 kb on + strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.845 kb on - strand, within BPHYT_RS00810 at 183.959 kb on - strand, within BPHYT_RS00810 at 184.009 kb on + strand, within BPHYT_RS00810 at 184.010 kb on - strand, within BPHYT_RS00810 at 184.140 kb on + strand at 184.141 kb on - strand at 184.141 kb on - strand at 184.584 kb on + strand, within BPHYT_RS00815 at 184.745 kb on + strand at 184.813 kb on + strand at 184.957 kb on + strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.958 kb on - strand, within BPHYT_RS00820 at 184.967 kb on + strand, within BPHYT_RS00820 at 185.225 kb on + strand, within BPHYT_RS00820 at 185.226 kb on - strand, within BPHYT_RS00820
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 182,182 - BPHYT_RS00805 0.21 -0.4 182,242 + +0.9 182,292 + BPHYT_RS00805 0.31 -0.1 182,339 + BPHYT_RS00805 0.34 +0.5 182,340 - BPHYT_RS00805 0.34 -1.0 182,340 - BPHYT_RS00805 0.34 +0.3 182,343 - BPHYT_RS00805 0.35 +0.2 182,635 + BPHYT_RS00805 0.59 -1.3 182,844 - BPHYT_RS00805 0.76 -1.2 182,844 - BPHYT_RS00805 0.76 -0.4 182,958 - BPHYT_RS00805 0.86 -0.4 182,958 - BPHYT_RS00805 0.86 +0.7 183,098 + +0.5 183,099 - -0.3 183,099 - -0.5 183,171 + -0.0 183,172 - +1.9 183,638 - BPHYT_RS00810 0.46 +0.2 183,697 - BPHYT_RS00810 0.51 -0.9 183,714 + BPHYT_RS00810 0.52 +1.4 183,844 + BPHYT_RS00810 0.64 -0.1 183,845 - BPHYT_RS00810 0.64 -0.4 183,845 - BPHYT_RS00810 0.64 -1.1 183,959 - BPHYT_RS00810 0.74 -1.3 184,009 + BPHYT_RS00810 0.78 -0.8 184,010 - BPHYT_RS00810 0.79 -0.2 184,140 + -0.2 184,141 - -2.4 184,141 - -0.8 184,584 + BPHYT_RS00815 0.77 -1.8 184,745 + -0.2 184,813 + +0.5 184,957 + BPHYT_RS00820 0.16 -0.9 184,958 - BPHYT_RS00820 0.16 +2.1 184,958 - BPHYT_RS00820 0.16 +0.6 184,967 + BPHYT_RS00820 0.16 -0.9 185,225 + BPHYT_RS00820 0.31 -1.6 185,226 - BPHYT_RS00820 0.31 -0.7
Or see this region's nucleotide sequence