Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00545 and BPHYT_RS00550 are separated by 301 nucleotides BPHYT_RS00550 and BPHYT_RS00555 are separated by 772 nucleotides
BPHYT_RS00545: BPHYT_RS00545 - LysR family transcriptional regulator, at 131,514 to 132,455
_RS00545
BPHYT_RS00550: BPHYT_RS00550 - excinuclease ABC subunit B, at 132,757 to 134,736
_RS00550
BPHYT_RS00555: BPHYT_RS00555 - hypothetical protein, at 135,509 to 135,910
_RS00555
Position (kb) 132
133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 131.777 kb on + strand, within BPHYT_RS00545 at 131.804 kb on - strand, within BPHYT_RS00545 at 131.804 kb on - strand, within BPHYT_RS00545 at 131.880 kb on - strand, within BPHYT_RS00545 at 131.949 kb on + strand, within BPHYT_RS00545 at 132.047 kb on + strand, within BPHYT_RS00545 at 132.047 kb on + strand, within BPHYT_RS00545 at 132.148 kb on - strand, within BPHYT_RS00545 at 132.443 kb on + strand at 132.516 kb on + strand at 132.526 kb on + strand at 132.537 kb on - strand at 132.608 kb on - strand at 132.608 kb on - strand at 132.610 kb on - strand at 132.610 kb on - strand at 132.768 kb on + strand at 132.792 kb on + strand at 132.793 kb on - strand at 132.843 kb on - strand at 132.901 kb on - strand at 132.979 kb on + strand, within BPHYT_RS00550 at 133.384 kb on + strand, within BPHYT_RS00550 at 133.385 kb on - strand, within BPHYT_RS00550 at 133.386 kb on + strand, within BPHYT_RS00550 at 133.387 kb on - strand, within BPHYT_RS00550 at 133.481 kb on - strand, within BPHYT_RS00550 at 133.481 kb on - strand, within BPHYT_RS00550 at 133.547 kb on + strand, within BPHYT_RS00550 at 133.685 kb on - strand, within BPHYT_RS00550 at 133.685 kb on - strand, within BPHYT_RS00550 at 133.694 kb on - strand, within BPHYT_RS00550 at 133.741 kb on + strand, within BPHYT_RS00550 at 133.810 kb on + strand, within BPHYT_RS00550 at 133.813 kb on - strand, within BPHYT_RS00550 at 133.813 kb on - strand, within BPHYT_RS00550 at 134.052 kb on + strand, within BPHYT_RS00550 at 134.052 kb on + strand, within BPHYT_RS00550 at 134.053 kb on - strand, within BPHYT_RS00550 at 134.199 kb on - strand, within BPHYT_RS00550 at 134.819 kb on + strand at 134.996 kb on + strand at 134.997 kb on - strand at 134.997 kb on - strand at 135.114 kb on - strand at 135.120 kb on + strand at 135.121 kb on - strand at 135.121 kb on - strand at 135.121 kb on - strand at 135.121 kb on - strand at 135.124 kb on + strand at 135.124 kb on + strand at 135.124 kb on + strand at 135.125 kb on - strand at 135.125 kb on - strand at 135.125 kb on - strand at 135.125 kb on - strand at 135.459 kb on - strand at 135.463 kb on - strand at 135.526 kb on - strand at 135.558 kb on + strand, within BPHYT_RS00555 at 135.558 kb on + strand, within BPHYT_RS00555 at 135.559 kb on - strand, within BPHYT_RS00555 at 135.665 kb on - strand, within BPHYT_RS00555 at 135.681 kb on + strand, within BPHYT_RS00555 at 135.693 kb on - strand, within BPHYT_RS00555 at 135.693 kb on - strand, within BPHYT_RS00555 at 135.693 kb on - strand, within BPHYT_RS00555
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 131,777 + BPHYT_RS00545 0.28 +0.3 131,804 - BPHYT_RS00545 0.31 +0.3 131,804 - BPHYT_RS00545 0.31 -0.2 131,880 - BPHYT_RS00545 0.39 +0.9 131,949 + BPHYT_RS00545 0.46 +1.0 132,047 + BPHYT_RS00545 0.57 -0.5 132,047 + BPHYT_RS00545 0.57 +1.8 132,148 - BPHYT_RS00545 0.67 +0.1 132,443 + -2.2 132,516 + -1.0 132,526 + +0.6 132,537 - +0.4 132,608 - -2.3 132,608 - +0.0 132,610 - +0.4 132,610 - -0.7 132,768 + +0.2 132,792 + +0.3 132,793 - -0.3 132,843 - +0.2 132,901 - +1.1 132,979 + BPHYT_RS00550 0.11 -0.8 133,384 + BPHYT_RS00550 0.32 -0.9 133,385 - BPHYT_RS00550 0.32 -0.3 133,386 + BPHYT_RS00550 0.32 -0.3 133,387 - BPHYT_RS00550 0.32 -0.1 133,481 - BPHYT_RS00550 0.37 -0.9 133,481 - BPHYT_RS00550 0.37 -0.9 133,547 + BPHYT_RS00550 0.40 -2.0 133,685 - BPHYT_RS00550 0.47 -1.6 133,685 - BPHYT_RS00550 0.47 -1.4 133,694 - BPHYT_RS00550 0.47 -0.8 133,741 + BPHYT_RS00550 0.50 +1.9 133,810 + BPHYT_RS00550 0.53 -0.4 133,813 - BPHYT_RS00550 0.53 -0.1 133,813 - BPHYT_RS00550 0.53 -0.5 134,052 + BPHYT_RS00550 0.65 +0.2 134,052 + BPHYT_RS00550 0.65 -0.1 134,053 - BPHYT_RS00550 0.65 +0.2 134,199 - BPHYT_RS00550 0.73 -2.2 134,819 + -0.7 134,996 + -1.3 134,997 - -0.1 134,997 - +4.2 135,114 - -1.0 135,120 + +0.3 135,121 - +0.1 135,121 - +0.1 135,121 - +1.7 135,121 - +2.1 135,124 + -0.8 135,124 + -1.9 135,124 + -0.0 135,125 - +0.5 135,125 - -2.0 135,125 - -0.7 135,125 - +0.3 135,459 - +1.5 135,463 - +1.4 135,526 - -2.1 135,558 + BPHYT_RS00555 0.12 -0.4 135,558 + BPHYT_RS00555 0.12 -0.5 135,559 - BPHYT_RS00555 0.12 +0.1 135,665 - BPHYT_RS00555 0.39 -0.7 135,681 + BPHYT_RS00555 0.43 +0.9 135,693 - BPHYT_RS00555 0.46 +0.5 135,693 - BPHYT_RS00555 0.46 -0.1 135,693 - BPHYT_RS00555 0.46 -2.0
Or see this region's nucleotide sequence