Experiment: 24hr PHYTO7 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34980 and BPHYT_RS34985 are separated by 26 nucleotides BPHYT_RS34985 and BPHYT_RS34990 overlap by 8 nucleotides BPHYT_RS34990 and BPHYT_RS34995 are separated by 25 nucleotides
BPHYT_RS34980: BPHYT_RS34980 - aldehyde dehydrogenase, at 3,446,907 to 3,448,397
_RS34980
BPHYT_RS34985: BPHYT_RS34985 - glyoxalase, at 3,448,424 to 3,449,389
_RS34985
BPHYT_RS34990: BPHYT_RS34990 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,449,382 to 3,450,647
_RS34990
BPHYT_RS34995: BPHYT_RS34995 - MFS transporter, at 3,450,673 to 3,452,076
_RS34995
Position (kb) 3449
3450
3451 Strain fitness (log2 ratio) -2
-1
0
1 at 3448.395 kb on + strand at 3448.854 kb on - strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985 at 3449.553 kb on + strand, within BPHYT_RS34990 at 3449.554 kb on - strand, within BPHYT_RS34990 at 3450.653 kb on + strand at 3450.656 kb on - strand at 3450.657 kb on - strand at 3451.050 kb on + strand, within BPHYT_RS34995 at 3451.579 kb on + strand, within BPHYT_RS34995
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10 remove 3,448,395 + -2.5 3,448,854 - BPHYT_RS34985 0.45 -2.1 3,449,113 + BPHYT_RS34985 0.71 -0.3 3,449,113 + BPHYT_RS34985 0.71 +0.2 3,449,553 + BPHYT_RS34990 0.14 -0.7 3,449,554 - BPHYT_RS34990 0.14 -0.9 3,450,653 + +0.3 3,450,656 - -0.3 3,450,657 - -0.4 3,451,050 + BPHYT_RS34995 0.27 +1.4 3,451,579 + BPHYT_RS34995 0.65 -1.5
Or see this region's nucleotide sequence