Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33135

Experiment: 24hr PHYTO7 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33130 and BPHYT_RS33135 are separated by 130 nucleotidesBPHYT_RS33135 and BPHYT_RS33140 are separated by 110 nucleotides BPHYT_RS33130: BPHYT_RS33130 - transcriptional regulator, at 3,010,016 to 3,011,428 _RS33130 BPHYT_RS33135: BPHYT_RS33135 - LysR family transcriptional regulator, at 3,011,559 to 3,012,446 _RS33135 BPHYT_RS33140: BPHYT_RS33140 - short-chain dehydrogenase, at 3,012,557 to 3,013,306 _RS33140 Position (kb) 3011 3012 3013Strain fitness (log2 ratio) -2 -1 0 1at 3010.677 kb on + strand, within BPHYT_RS33130at 3010.805 kb on + strand, within BPHYT_RS33130at 3010.995 kb on - strand, within BPHYT_RS33130at 3011.320 kb on - strandat 3011.320 kb on - strandat 3011.320 kb on - strandat 3011.322 kb on + strandat 3011.322 kb on + strandat 3011.412 kb on - strandat 3011.624 kb on + strandat 3011.932 kb on + strand, within BPHYT_RS33135at 3012.223 kb on - strand, within BPHYT_RS33135at 3012.244 kb on + strand, within BPHYT_RS33135at 3012.447 kb on + strandat 3012.504 kb on + strandat 3012.507 kb on - strandat 3012.507 kb on - strandat 3012.806 kb on + strand, within BPHYT_RS33140at 3013.319 kb on + strandat 3013.427 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10
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3,010,677 + BPHYT_RS33130 0.47 +0.1
3,010,805 + BPHYT_RS33130 0.56 +0.8
3,010,995 - BPHYT_RS33130 0.69 -0.3
3,011,320 - -0.4
3,011,320 - +1.1
3,011,320 - -0.5
3,011,322 + -0.7
3,011,322 + -1.5
3,011,412 - -0.2
3,011,624 + +0.2
3,011,932 + BPHYT_RS33135 0.42 +1.2
3,012,223 - BPHYT_RS33135 0.75 +0.1
3,012,244 + BPHYT_RS33135 0.77 +0.3
3,012,447 + +1.0
3,012,504 + -1.4
3,012,507 - +0.1
3,012,507 - +1.3
3,012,806 + BPHYT_RS33140 0.33 -2.2
3,013,319 + -1.1
3,013,427 + -1.6

Or see this region's nucleotide sequence