Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31470

Experiment: 24hr PHYTO7 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31465 and BPHYT_RS31470 are separated by 182 nucleotidesBPHYT_RS31470 and BPHYT_RS31475 are separated by 537 nucleotides BPHYT_RS31465: BPHYT_RS31465 - D-isomer specific 2-hydroxyacid dehydrogenase, at 2,612,457 to 2,613,281 _RS31465 BPHYT_RS31470: BPHYT_RS31470 - NAD(P)H dehydrogenase (quinone), at 2,613,464 to 2,614,066 _RS31470 BPHYT_RS31475: BPHYT_RS31475 - hypothetical protein, at 2,614,604 to 2,615,428 _RS31475 Position (kb) 2613 2614 2615Strain fitness (log2 ratio) -2 -1 0 1 2at 2612.703 kb on + strand, within BPHYT_RS31465at 2612.908 kb on - strand, within BPHYT_RS31465at 2613.365 kb on + strandat 2613.521 kb on - strandat 2613.665 kb on + strand, within BPHYT_RS31470at 2613.665 kb on + strand, within BPHYT_RS31470at 2614.214 kb on + strandat 2614.475 kb on + strandat 2614.476 kb on - strandat 2614.501 kb on + strandat 2614.585 kb on + strandat 2614.585 kb on + strandat 2614.586 kb on - strandat 2614.896 kb on + strand, within BPHYT_RS31475at 2614.958 kb on + strand, within BPHYT_RS31475

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10
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2,612,703 + BPHYT_RS31465 0.30 +0.1
2,612,908 - BPHYT_RS31465 0.55 +1.4
2,613,365 + -0.0
2,613,521 - +2.0
2,613,665 + BPHYT_RS31470 0.33 -2.4
2,613,665 + BPHYT_RS31470 0.33 -0.7
2,614,214 + -1.0
2,614,475 + +0.8
2,614,476 - -0.2
2,614,501 + +0.2
2,614,585 + -0.4
2,614,585 + -1.0
2,614,586 - -0.4
2,614,896 + BPHYT_RS31475 0.35 -0.5
2,614,958 + BPHYT_RS31475 0.43 -0.3

Or see this region's nucleotide sequence