Experiment: 24hr PHYTO7 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30195 and BPHYT_RS30200 are separated by 79 nucleotides BPHYT_RS30200 and BPHYT_RS30205 are separated by 43 nucleotides
BPHYT_RS30195: BPHYT_RS30195 - ABC transporter substrate-binding protein, at 2,328,450 to 2,330,066
_RS30195
BPHYT_RS30200: BPHYT_RS30200 - FAD-dependent oxidoreductase, at 2,330,146 to 2,331,420
_RS30200
BPHYT_RS30205: BPHYT_RS30205 - dehalogenase, at 2,331,464 to 2,332,132
_RS30205
Position (kb)
2330
2331
2332 Strain fitness (log2 ratio)
-2
-1
0
1 at 2329.778 kb on - strand, within BPHYT_RS30195 at 2329.799 kb on - strand, within BPHYT_RS30195 at 2330.174 kb on + strand at 2330.174 kb on + strand at 2330.175 kb on - strand at 2330.345 kb on + strand, within BPHYT_RS30200 at 2330.414 kb on + strand, within BPHYT_RS30200 at 2331.069 kb on - strand, within BPHYT_RS30200 at 2331.069 kb on - strand, within BPHYT_RS30200 at 2331.465 kb on + strand at 2331.510 kb on + strand at 2331.514 kb on - strand at 2331.519 kb on + strand at 2331.522 kb on + strand at 2331.523 kb on - strand at 2331.663 kb on + strand, within BPHYT_RS30205 at 2331.663 kb on + strand, within BPHYT_RS30205 at 2331.722 kb on - strand, within BPHYT_RS30205 at 2331.798 kb on + strand, within BPHYT_RS30205 at 2332.111 kb on - strand at 2332.111 kb on - strand at 2332.230 kb on + strand at 2332.383 kb on + strand at 2332.383 kb on + strand at 2332.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10 remove 2,329,778 - BPHYT_RS30195 0.82 -0.4 2,329,799 - BPHYT_RS30195 0.83 +0.4 2,330,174 + -0.7 2,330,174 + +1.5 2,330,175 - +0.6 2,330,345 + BPHYT_RS30200 0.16 -2.3 2,330,414 + BPHYT_RS30200 0.21 -0.4 2,331,069 - BPHYT_RS30200 0.72 +0.5 2,331,069 - BPHYT_RS30200 0.72 +0.5 2,331,465 + +0.2 2,331,510 + +0.3 2,331,514 - -1.3 2,331,519 + +1.3 2,331,522 + -1.4 2,331,523 - +0.5 2,331,663 + BPHYT_RS30205 0.30 +0.4 2,331,663 + BPHYT_RS30205 0.30 -0.8 2,331,722 - BPHYT_RS30205 0.39 +0.5 2,331,798 + BPHYT_RS30205 0.50 +0.4 2,332,111 - -0.2 2,332,111 - -0.1 2,332,230 + +0.3 2,332,383 + +0.4 2,332,383 + -0.5 2,332,384 - -0.9
Or see this region's nucleotide sequence