Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23525

Experiment: 24hr PHYTO7 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23520 and BPHYT_RS23525 are separated by 202 nucleotidesBPHYT_RS23525 and BPHYT_RS23530 are separated by 512 nucleotidesBPHYT_RS23530 and BPHYT_RS23535 are separated by 190 nucleotides BPHYT_RS23520: BPHYT_RS23520 - peroxidase, at 844,433 to 845,500 _RS23520 BPHYT_RS23525: BPHYT_RS23525 - hypothetical protein, at 845,703 to 846,050 _RS23525 BPHYT_RS23530: BPHYT_RS23530 - hypothetical protein, at 846,563 to 846,790 _RS23530 BPHYT_RS23535: BPHYT_RS23535 - ABC transporter substrate-binding protein, at 846,981 to 847,700 _RS23535 Position (kb) 845 846 847Strain fitness (log2 ratio) -1 0 1 2at 844.771 kb on - strand, within BPHYT_RS23520at 845.224 kb on - strand, within BPHYT_RS23520at 845.704 kb on + strandat 845.709 kb on + strandat 846.007 kb on - strand, within BPHYT_RS23525at 846.342 kb on + strandat 846.414 kb on + strandat 846.453 kb on - strandat 846.487 kb on + strandat 846.877 kb on + strandat 846.882 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10
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844,771 - BPHYT_RS23520 0.32 -0.6
845,224 - BPHYT_RS23520 0.74 -1.4
845,704 + +0.0
845,709 + +2.5
846,007 - BPHYT_RS23525 0.87 -1.3
846,342 + +0.6
846,414 + +1.4
846,453 - -0.1
846,487 + -0.3
846,877 + +1.2
846,882 - -0.4

Or see this region's nucleotide sequence