Experiment: 24hr PHYTO7 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23260 and BPHYT_RS23265 are separated by 49 nucleotides BPHYT_RS23265 and BPHYT_RS23270 are separated by 41 nucleotides BPHYT_RS23270 and BPHYT_RS23275 are separated by 81 nucleotides
BPHYT_RS23260: BPHYT_RS23260 - isovaleryl-CoA dehydrogenase, at 782,334 to 783,515
_RS23260
BPHYT_RS23265: BPHYT_RS23265 - methylcrotonoyl-CoA carboxylase, at 783,565 to 785,172
_RS23265
BPHYT_RS23270: BPHYT_RS23270 - enoyl-CoA hydratase, at 785,214 to 785,999
_RS23270
BPHYT_RS23275: BPHYT_RS23275 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 786,081 to 788,159
_RS23275
Position (kb) 783
784
785
786 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 782.680 kb on - strand, within BPHYT_RS23260 at 782.680 kb on - strand, within BPHYT_RS23260 at 782.793 kb on + strand, within BPHYT_RS23260 at 782.794 kb on - strand, within BPHYT_RS23260 at 783.142 kb on - strand, within BPHYT_RS23260 at 783.217 kb on - strand, within BPHYT_RS23260 at 783.217 kb on - strand, within BPHYT_RS23260 at 783.331 kb on - strand, within BPHYT_RS23260 at 783.394 kb on - strand, within BPHYT_RS23260 at 783.442 kb on - strand at 783.563 kb on + strand at 783.564 kb on - strand at 783.808 kb on + strand, within BPHYT_RS23265 at 783.809 kb on - strand, within BPHYT_RS23265 at 783.913 kb on + strand, within BPHYT_RS23265 at 784.068 kb on + strand, within BPHYT_RS23265 at 784.144 kb on + strand, within BPHYT_RS23265 at 784.278 kb on + strand, within BPHYT_RS23265 at 784.433 kb on - strand, within BPHYT_RS23265 at 785.658 kb on + strand, within BPHYT_RS23270
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10 remove 782,680 - BPHYT_RS23260 0.29 -1.2 782,680 - BPHYT_RS23260 0.29 +0.8 782,793 + BPHYT_RS23260 0.39 +0.4 782,794 - BPHYT_RS23260 0.39 -1.3 783,142 - BPHYT_RS23260 0.68 -0.8 783,217 - BPHYT_RS23260 0.75 -0.3 783,217 - BPHYT_RS23260 0.75 -0.4 783,331 - BPHYT_RS23260 0.84 -0.1 783,394 - BPHYT_RS23260 0.90 -1.6 783,442 - +1.6 783,563 + -0.2 783,564 - +0.2 783,808 + BPHYT_RS23265 0.15 -3.0 783,809 - BPHYT_RS23265 0.15 +2.0 783,913 + BPHYT_RS23265 0.22 +1.1 784,068 + BPHYT_RS23265 0.31 -0.2 784,144 + BPHYT_RS23265 0.36 +0.5 784,278 + BPHYT_RS23265 0.44 +1.4 784,433 - BPHYT_RS23265 0.54 +1.4 785,658 + BPHYT_RS23270 0.56 -0.7
Or see this region's nucleotide sequence