Experiment: 24hr PHYTO7 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20375 and BPHYT_RS20380 are separated by 127 nucleotides BPHYT_RS20380 and BPHYT_RS20385 are separated by 109 nucleotides BPHYT_RS20385 and BPHYT_RS20390 are separated by 78 nucleotides
BPHYT_RS20375: BPHYT_RS20375 - NADH-flavin oxidoreductase / NADH oxidase, at 133,904 to 135,013
_RS20375
BPHYT_RS20380: BPHYT_RS20380 - LysR family transcriptional regulator, at 135,141 to 136,067
_RS20380
BPHYT_RS20385: BPHYT_RS20385 - TetR family transcriptional regulator, at 136,177 to 136,761
_RS20385
BPHYT_RS20390: BPHYT_RS20390 - oxidoreductase, at 136,840 to 137,961
_RS20390
Position (kb) 135
136
137 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 134.204 kb on + strand, within BPHYT_RS20375 at 134.295 kb on - strand, within BPHYT_RS20375 at 134.489 kb on - strand, within BPHYT_RS20375 at 134.492 kb on + strand, within BPHYT_RS20375 at 134.492 kb on + strand, within BPHYT_RS20375 at 134.492 kb on + strand, within BPHYT_RS20375 at 134.612 kb on - strand, within BPHYT_RS20375 at 134.641 kb on + strand, within BPHYT_RS20375 at 134.645 kb on + strand, within BPHYT_RS20375 at 134.681 kb on + strand, within BPHYT_RS20375 at 134.681 kb on + strand, within BPHYT_RS20375 at 134.775 kb on - strand, within BPHYT_RS20375 at 134.931 kb on - strand at 134.987 kb on + strand at 135.011 kb on + strand at 135.027 kb on - strand at 135.088 kb on - strand at 135.208 kb on + strand at 135.231 kb on - strand at 135.336 kb on + strand, within BPHYT_RS20380 at 136.011 kb on - strand at 136.125 kb on - strand at 136.208 kb on - strand at 136.208 kb on - strand at 136.358 kb on + strand, within BPHYT_RS20385 at 136.360 kb on + strand, within BPHYT_RS20385 at 136.360 kb on + strand, within BPHYT_RS20385 at 136.360 kb on + strand, within BPHYT_RS20385 at 136.360 kb on + strand, within BPHYT_RS20385 at 136.361 kb on - strand, within BPHYT_RS20385 at 136.505 kb on + strand, within BPHYT_RS20385 at 136.583 kb on + strand, within BPHYT_RS20385 at 136.782 kb on + strand at 136.855 kb on - strand at 137.030 kb on + strand, within BPHYT_RS20390
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10 remove 134,204 + BPHYT_RS20375 0.27 +0.0 134,295 - BPHYT_RS20375 0.35 -0.1 134,489 - BPHYT_RS20375 0.53 -1.2 134,492 + BPHYT_RS20375 0.53 +0.0 134,492 + BPHYT_RS20375 0.53 -0.5 134,492 + BPHYT_RS20375 0.53 +0.7 134,612 - BPHYT_RS20375 0.64 -0.9 134,641 + BPHYT_RS20375 0.66 -0.6 134,645 + BPHYT_RS20375 0.67 +0.3 134,681 + BPHYT_RS20375 0.70 -0.5 134,681 + BPHYT_RS20375 0.70 -0.1 134,775 - BPHYT_RS20375 0.78 +0.0 134,931 - -0.6 134,987 + -0.0 135,011 + +2.4 135,027 - -0.4 135,088 - -1.4 135,208 + +0.8 135,231 - +0.7 135,336 + BPHYT_RS20380 0.21 -1.3 136,011 - -0.2 136,125 - -0.4 136,208 - -0.8 136,208 - +1.3 136,358 + BPHYT_RS20385 0.31 +0.1 136,360 + BPHYT_RS20385 0.31 +0.2 136,360 + BPHYT_RS20385 0.31 -0.5 136,360 + BPHYT_RS20385 0.31 -1.6 136,360 + BPHYT_RS20385 0.31 -2.7 136,361 - BPHYT_RS20385 0.31 -0.4 136,505 + BPHYT_RS20385 0.56 -0.5 136,583 + BPHYT_RS20385 0.69 -0.8 136,782 + +0.2 136,855 - -2.9 137,030 + BPHYT_RS20390 0.17 -0.8
Or see this region's nucleotide sequence