Experiment: 24hr PHYTO7 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07210 and BPHYT_RS07215 are separated by 107 nucleotides BPHYT_RS07215 and BPHYT_RS07220 are separated by 105 nucleotides BPHYT_RS07220 and BPHYT_RS07225 are separated by 211 nucleotides
BPHYT_RS07210: BPHYT_RS07210 - permease, at 1,638,851 to 1,639,741
_RS07210
BPHYT_RS07215: BPHYT_RS07215 - LysR family transcriptional regulator, at 1,639,849 to 1,640,754
_RS07215
BPHYT_RS07220: BPHYT_RS07220 - GCN5 family acetyltransferase, at 1,640,860 to 1,641,489
_RS07220
BPHYT_RS07225: BPHYT_RS07225 - membrane protein, at 1,641,701 to 1,641,862
_RS07225
Position (kb) 1639
1640
1641 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1639.219 kb on - strand, within BPHYT_RS07210 at 1639.219 kb on - strand, within BPHYT_RS07210 at 1639.591 kb on - strand, within BPHYT_RS07210 at 1639.792 kb on - strand at 1639.807 kb on + strand at 1639.812 kb on + strand at 1639.812 kb on + strand at 1639.813 kb on - strand at 1639.820 kb on + strand at 1640.073 kb on - strand, within BPHYT_RS07215 at 1640.327 kb on + strand, within BPHYT_RS07215 at 1640.327 kb on + strand, within BPHYT_RS07215 at 1640.895 kb on + strand at 1640.902 kb on + strand at 1640.903 kb on - strand at 1641.161 kb on + strand, within BPHYT_RS07220 at 1641.162 kb on - strand, within BPHYT_RS07220
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 10 remove 1,639,219 - BPHYT_RS07210 0.41 -0.3 1,639,219 - BPHYT_RS07210 0.41 -0.4 1,639,591 - BPHYT_RS07210 0.83 -1.4 1,639,792 - +1.0 1,639,807 + -0.4 1,639,812 + +0.1 1,639,812 + -1.4 1,639,813 - -1.3 1,639,820 + -1.6 1,640,073 - BPHYT_RS07215 0.25 -2.9 1,640,327 + BPHYT_RS07215 0.53 -1.0 1,640,327 + BPHYT_RS07215 0.53 +1.5 1,640,895 + -0.5 1,640,902 + +1.2 1,640,903 - +0.6 1,641,161 + BPHYT_RS07220 0.48 +0.6 1,641,162 - BPHYT_RS07220 0.48 -1.0
Or see this region's nucleotide sequence