Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32135

Experiment: 24hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32125 and BPHYT_RS32130 are separated by 55 nucleotidesBPHYT_RS32130 and BPHYT_RS32135 overlap by 4 nucleotidesBPHYT_RS32135 and BPHYT_RS32140 are separated by 51 nucleotides BPHYT_RS32125: BPHYT_RS32125 - DNA polymerase, at 2,780,146 to 2,781,159 _RS32125 BPHYT_RS32130: BPHYT_RS32130 - hypothetical protein, at 2,781,215 to 2,781,709 _RS32130 BPHYT_RS32135: BPHYT_RS32135 - DNA primase, at 2,781,706 to 2,783,229 _RS32135 BPHYT_RS32140: BPHYT_RS32140 - DNA replication protein, at 2,783,281 to 2,785,146 _RS32140 Position (kb) 2781 2782 2783 2784Strain fitness (log2 ratio) -1 0 1 2at 2780.779 kb on + strand, within BPHYT_RS32125at 2780.848 kb on + strand, within BPHYT_RS32125at 2780.849 kb on - strand, within BPHYT_RS32125at 2780.916 kb on + strand, within BPHYT_RS32125at 2780.916 kb on + strand, within BPHYT_RS32125at 2780.917 kb on - strand, within BPHYT_RS32125at 2780.986 kb on + strand, within BPHYT_RS32125at 2780.987 kb on - strand, within BPHYT_RS32125at 2781.278 kb on + strand, within BPHYT_RS32130at 2781.278 kb on + strand, within BPHYT_RS32130at 2781.278 kb on + strand, within BPHYT_RS32130at 2781.279 kb on - strand, within BPHYT_RS32130at 2781.698 kb on - strandat 2781.708 kb on - strandat 2782.115 kb on - strand, within BPHYT_RS32135at 2782.162 kb on + strand, within BPHYT_RS32135at 2782.163 kb on - strand, within BPHYT_RS32135at 2782.163 kb on - strand, within BPHYT_RS32135at 2782.163 kb on - strand, within BPHYT_RS32135at 2782.186 kb on + strand, within BPHYT_RS32135at 2782.328 kb on + strand, within BPHYT_RS32135at 2782.328 kb on + strand, within BPHYT_RS32135at 2782.328 kb on + strand, within BPHYT_RS32135at 2782.398 kb on - strand, within BPHYT_RS32135at 2782.564 kb on + strand, within BPHYT_RS32135at 2782.564 kb on + strand, within BPHYT_RS32135at 2782.564 kb on + strand, within BPHYT_RS32135at 2782.565 kb on - strand, within BPHYT_RS32135at 2782.565 kb on - strand, within BPHYT_RS32135at 2782.570 kb on + strand, within BPHYT_RS32135at 2782.571 kb on - strand, within BPHYT_RS32135at 2782.717 kb on + strand, within BPHYT_RS32135at 2782.718 kb on - strand, within BPHYT_RS32135at 2782.883 kb on + strand, within BPHYT_RS32135at 2783.663 kb on - strand, within BPHYT_RS32140at 2783.710 kb on + strand, within BPHYT_RS32140at 2783.746 kb on + strand, within BPHYT_RS32140at 2783.747 kb on - strand, within BPHYT_RS32140at 2784.058 kb on + strand, within BPHYT_RS32140at 2784.058 kb on + strand, within BPHYT_RS32140

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr Phyto Lib only
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2,780,779 + BPHYT_RS32125 0.62 +1.1
2,780,848 + BPHYT_RS32125 0.69 +0.8
2,780,849 - BPHYT_RS32125 0.69 -0.8
2,780,916 + BPHYT_RS32125 0.76 -0.7
2,780,916 + BPHYT_RS32125 0.76 -1.1
2,780,917 - BPHYT_RS32125 0.76 +1.1
2,780,986 + BPHYT_RS32125 0.83 -0.7
2,780,987 - BPHYT_RS32125 0.83 -0.2
2,781,278 + BPHYT_RS32130 0.13 -0.3
2,781,278 + BPHYT_RS32130 0.13 +0.5
2,781,278 + BPHYT_RS32130 0.13 +0.1
2,781,279 - BPHYT_RS32130 0.13 +0.1
2,781,698 - -0.1
2,781,708 - -0.3
2,782,115 - BPHYT_RS32135 0.27 -0.5
2,782,162 + BPHYT_RS32135 0.30 -0.5
2,782,163 - BPHYT_RS32135 0.30 -0.7
2,782,163 - BPHYT_RS32135 0.30 -1.2
2,782,163 - BPHYT_RS32135 0.30 -0.5
2,782,186 + BPHYT_RS32135 0.31 +1.0
2,782,328 + BPHYT_RS32135 0.41 +0.4
2,782,328 + BPHYT_RS32135 0.41 +2.1
2,782,328 + BPHYT_RS32135 0.41 -0.8
2,782,398 - BPHYT_RS32135 0.45 -0.7
2,782,564 + BPHYT_RS32135 0.56 -0.9
2,782,564 + BPHYT_RS32135 0.56 -0.1
2,782,564 + BPHYT_RS32135 0.56 -1.0
2,782,565 - BPHYT_RS32135 0.56 -0.1
2,782,565 - BPHYT_RS32135 0.56 -0.2
2,782,570 + BPHYT_RS32135 0.57 -0.3
2,782,571 - BPHYT_RS32135 0.57 +1.1
2,782,717 + BPHYT_RS32135 0.66 +0.1
2,782,718 - BPHYT_RS32135 0.66 -0.6
2,782,883 + BPHYT_RS32135 0.77 -1.2
2,783,663 - BPHYT_RS32140 0.20 +0.3
2,783,710 + BPHYT_RS32140 0.23 -0.4
2,783,746 + BPHYT_RS32140 0.25 +0.5
2,783,747 - BPHYT_RS32140 0.25 +0.7
2,784,058 + BPHYT_RS32140 0.42 +0.6
2,784,058 + BPHYT_RS32140 0.42 +0.8

Or see this region's nucleotide sequence