Experiment: 24hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27710 and BPHYT_RS27715 are separated by 173 nucleotides BPHYT_RS27715 and BPHYT_RS27720 overlap by 2 nucleotides BPHYT_RS27720 and BPHYT_RS27725 are separated by 0 nucleotides BPHYT_RS27725 and BPHYT_RS27730 are separated by 46 nucleotides BPHYT_RS27730 and BPHYT_RS27735 are separated by 171 nucleotides
BPHYT_RS27710: BPHYT_RS27710 - universal stress protein A, at 1,782,113 to 1,782,943
_RS27710
BPHYT_RS27715: BPHYT_RS27715 - UspA domain-containing protein, at 1,783,117 to 1,783,593
_RS27715
BPHYT_RS27720: BPHYT_RS27720 - hypothetical protein, at 1,783,592 to 1,783,831
_RS27720
BPHYT_RS27725: BPHYT_RS27725 - hypothetical protein, at 1,783,832 to 1,784,050
_RS27725
BPHYT_RS27730: BPHYT_RS27730 - LuxR family transcriptional regulator, at 1,784,097 to 1,784,744
_RS27730
BPHYT_RS27735: BPHYT_RS27735 - nitroreductase, at 1,784,916 to 1,785,947
_RS27735
Position (kb) 1783
1784
1785 Strain fitness (log2 ratio) -1
0
1 at 1783.014 kb on - strand at 1783.417 kb on - strand, within BPHYT_RS27715 at 1783.679 kb on - strand, within BPHYT_RS27720 at 1783.943 kb on - strand, within BPHYT_RS27725 at 1784.799 kb on + strand at 1784.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib only remove 1,783,014 - +0.5 1,783,417 - BPHYT_RS27715 0.63 +0.1 1,783,679 - BPHYT_RS27720 0.36 +0.8 1,783,943 - BPHYT_RS27725 0.51 +0.1 1,784,799 + -0.3 1,784,888 + -0.0
Or see this region's nucleotide sequence