Experiment: 24hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotides BPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotides BPHYT_RS20120 and BPHYT_RS20125 are separated by 396 nucleotides
BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868
_RS20110
BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592
_RS20115
BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899
_RS20120
BPHYT_RS20125: BPHYT_RS20125 - histidine kinase, at 58,296 to 58,511
_RS20125
Position (kb) 56
57
58 Strain fitness (log2 ratio) -2
-1
0
1 at 55.877 kb on + strand at 56.026 kb on + strand, within BPHYT_RS20115 at 56.469 kb on + strand, within BPHYT_RS20115 at 56.475 kb on + strand, within BPHYT_RS20115 at 56.625 kb on - strand at 56.930 kb on + strand, within BPHYT_RS20120 at 57.318 kb on + strand, within BPHYT_RS20120 at 57.528 kb on + strand, within BPHYT_RS20120 at 57.808 kb on - strand at 57.974 kb on + strand at 57.975 kb on - strand at 58.135 kb on - strand at 58.696 kb on - strand at 58.897 kb on + strand at 58.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr Phyto Lib only remove 55,877 + -1.0 56,026 + BPHYT_RS20115 0.19 -0.1 56,469 + BPHYT_RS20115 0.82 -0.4 56,475 + BPHYT_RS20115 0.83 -2.0 56,625 - +0.1 56,930 + BPHYT_RS20120 0.14 -0.9 57,318 + BPHYT_RS20120 0.48 -1.2 57,528 + BPHYT_RS20120 0.67 -0.7 57,808 - -0.2 57,974 + -1.3 57,975 - +0.2 58,135 - +1.2 58,696 - +1.0 58,897 + +0.5 58,898 - -1.2
Or see this region's nucleotide sequence