Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36380 and BPHYT_RS36385 overlap by 4 nucleotides BPHYT_RS36385 and BPHYT_RS36390 overlap by 4 nucleotides BPHYT_RS36390 and BPHYT_RS36395 are separated by 84 nucleotides
BPHYT_RS36380: BPHYT_RS36380 - phage integrase, at 88,818 to 90,884
_RS36380
BPHYT_RS36385: BPHYT_RS36385 - TetR family transcriptional regulator, at 90,881 to 91,273
_RS36385
BPHYT_RS36390: BPHYT_RS36390 - hypothetical protein, at 91,270 to 91,524
_RS36390
BPHYT_RS36395: BPHYT_RS36395 - hypothetical protein, at 91,609 to 91,920
_RS36395
Position (kb) 90
91
92 Strain fitness (log2 ratio) -1
0
1
2 at 90.002 kb on + strand, within BPHYT_RS36380 at 90.358 kb on - strand, within BPHYT_RS36380 at 90.358 kb on - strand at 90.848 kb on - strand at 91.173 kb on + strand, within BPHYT_RS36385 at 91.507 kb on + strand at 91.514 kb on - strand at 91.650 kb on + strand, within BPHYT_RS36395 at 91.651 kb on - strand, within BPHYT_RS36395 at 91.985 kb on - strand at 91.985 kb on - strand at 92.180 kb on + strand at 92.185 kb on - strand at 92.185 kb on - strand at 92.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 90,002 + BPHYT_RS36380 0.57 -0.5 90,358 - BPHYT_RS36380 0.75 -0.1 90,358 - +0.5 90,848 - -0.8 91,173 + BPHYT_RS36385 0.74 -0.9 91,507 + +2.1 91,514 - +0.1 91,650 + BPHYT_RS36395 0.13 +2.1 91,651 - BPHYT_RS36395 0.13 +0.5 91,985 - -0.0 91,985 - -0.4 92,180 + -1.0 92,185 - +0.5 92,185 - +1.1 92,190 + -0.1
Or see this region's nucleotide sequence