Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35980

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35970 and BPHYT_RS35975 are separated by 66 nucleotidesBPHYT_RS35975 and BPHYT_RS35980 are separated by 165 nucleotidesBPHYT_RS35980 and BPHYT_RS35985 are separated by 100 nucleotides BPHYT_RS35970: BPHYT_RS35970 - DNA recombinase, at 27,468 to 28,574 _RS35970 BPHYT_RS35975: BPHYT_RS35975 - Xre family transcriptional regulator, at 28,641 to 28,880 _RS35975 BPHYT_RS35980: BPHYT_RS35980 - hypothetical protein, at 29,046 to 30,128 _RS35980 BPHYT_RS35985: BPHYT_RS35985 - hypothetical protein, at 30,229 to 30,975 _RS35985 Position (kb) 29 30 31Strain fitness (log2 ratio) -2 -1 0 1 2at 28.316 kb on + strand, within BPHYT_RS35970at 28.418 kb on + strand, within BPHYT_RS35970at 28.441 kb on - strand, within BPHYT_RS35970at 28.441 kb on - strand, within BPHYT_RS35970at 28.446 kb on - strand, within BPHYT_RS35970at 28.553 kb on - strandat 28.579 kb on - strandat 28.770 kb on - strand, within BPHYT_RS35975at 28.771 kb on + strand, within BPHYT_RS35975at 28.771 kb on + strand, within BPHYT_RS35975at 28.771 kb on + strand, within BPHYT_RS35975at 28.771 kb on + strand, within BPHYT_RS35975at 28.771 kb on + strand, within BPHYT_RS35975at 28.772 kb on - strand, within BPHYT_RS35975at 28.772 kb on - strand, within BPHYT_RS35975at 28.772 kb on - strand, within BPHYT_RS35975at 28.827 kb on - strand, within BPHYT_RS35975at 28.834 kb on - strand, within BPHYT_RS35975at 28.891 kb on + strandat 28.892 kb on - strandat 28.895 kb on + strandat 28.927 kb on - strandat 28.927 kb on - strandat 28.942 kb on - strandat 28.957 kb on + strandat 28.957 kb on + strandat 29.045 kb on - strandat 29.111 kb on + strandat 29.111 kb on + strandat 29.111 kb on + strandat 29.112 kb on - strandat 29.112 kb on - strandat 29.112 kb on - strandat 29.114 kb on - strandat 29.114 kb on - strandat 29.513 kb on - strand, within BPHYT_RS35980at 29.599 kb on + strand, within BPHYT_RS35980at 29.600 kb on - strand, within BPHYT_RS35980at 29.656 kb on + strand, within BPHYT_RS35980at 29.656 kb on + strand, within BPHYT_RS35980at 29.657 kb on - strand, within BPHYT_RS35980at 29.657 kb on - strand, within BPHYT_RS35980at 29.673 kb on - strand, within BPHYT_RS35980at 30.794 kb on + strand, within BPHYT_RS35985at 30.795 kb on - strand, within BPHYT_RS35985at 30.803 kb on + strand, within BPHYT_RS35985at 30.803 kb on + strand, within BPHYT_RS35985at 31.108 kb on + strandat 31.108 kb on + strandat 31.109 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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28,316 + BPHYT_RS35970 0.77 -0.6
28,418 + BPHYT_RS35970 0.86 -1.2
28,441 - BPHYT_RS35970 0.88 +0.0
28,441 - BPHYT_RS35970 0.88 +0.3
28,446 - BPHYT_RS35970 0.88 -0.1
28,553 - -0.8
28,579 - +0.5
28,770 - BPHYT_RS35975 0.54 +0.5
28,771 + BPHYT_RS35975 0.54 -0.3
28,771 + BPHYT_RS35975 0.54 +1.2
28,771 + BPHYT_RS35975 0.54 +0.6
28,771 + BPHYT_RS35975 0.54 +1.7
28,771 + BPHYT_RS35975 0.54 -0.7
28,772 - BPHYT_RS35975 0.55 -0.9
28,772 - BPHYT_RS35975 0.55 -1.5
28,772 - BPHYT_RS35975 0.55 -0.4
28,827 - BPHYT_RS35975 0.78 +0.8
28,834 - BPHYT_RS35975 0.80 +0.7
28,891 + -0.9
28,892 - -1.5
28,895 + -0.4
28,927 - -1.9
28,927 - +0.2
28,942 - -0.2
28,957 + +0.1
28,957 + -1.2
29,045 - -0.4
29,111 + +0.1
29,111 + +0.5
29,111 + +0.8
29,112 - +0.3
29,112 - +0.1
29,112 - -1.2
29,114 - +0.0
29,114 - -0.4
29,513 - BPHYT_RS35980 0.43 -0.7
29,599 + BPHYT_RS35980 0.51 +0.4
29,600 - BPHYT_RS35980 0.51 +0.0
29,656 + BPHYT_RS35980 0.56 +2.5
29,656 + BPHYT_RS35980 0.56 +0.8
29,657 - BPHYT_RS35980 0.56 +0.0
29,657 - BPHYT_RS35980 0.56 -0.1
29,673 - BPHYT_RS35980 0.58 +0.5
30,794 + BPHYT_RS35985 0.76 -0.3
30,795 - BPHYT_RS35985 0.76 -0.4
30,803 + BPHYT_RS35985 0.77 +0.2
30,803 + BPHYT_RS35985 0.77 -0.6
31,108 + -0.2
31,108 + +1.1
31,109 - +1.1

Or see this region's nucleotide sequence